cDNA Clone E1a

• Plasmid: pEGFP-C1-E1a
• Parent Plasmid: pEGFPC1
• Expressed: GFP-E1a
• Genbank: P22314/X56976
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGACATA
GACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAAT
GAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGG
GCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACC
CTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTA
CCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCC
GCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGACCC
CTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAACAC
CCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGGCA
TCAAGCTGGTGGTGGCAGGCACGCGGGGCCTGTTTGGGCAGCTCTTCTGT
GACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACT
CAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCT
GCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCCTTT
TCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGAGAT
CAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACTTCT
CCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAG
ATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGT
GACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCTTCC
AGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCCCGC
AATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAATGC
TCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCATCC
GGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCCTTC
ATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTT
CATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTC
AGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAACCGT
TATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCTGGG
CAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGCTGC
TCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAAATC
ATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACAGTT
TCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTG
CAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAGAAC
CGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCAAAA
CCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGTACA
TGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACA
CTGGGCACCAAAGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGAGTC
GTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTACCC
TGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGGGAT
GAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTACCT
CACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTCAGC
CCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCA
GTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACCAGC
TCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCACAC
CCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGATGGC
TGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGGACC
GAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTCACC
CCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGC
CAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGC
CCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTTGAG
AAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATCCAA
CCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGAGCA
AGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCC
GTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCACCG
ACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCT
TTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAACCAA
GAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAATGG
TGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGCACA
AATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTC
TTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGA
GATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGGCGC
TGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAGGTT
CCCTATGTCCGATACACCATCCGCTGAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNG
MAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKN
IILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNS
YVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVAG
TRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARH
GFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGG
IVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFC
AQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVA
AGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPQDKEVLT
EDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMI
GLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMN
PHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVY
YRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNA
IEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEA
VQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAP
FWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATF
LQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP
GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKI
IPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPL
AAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITML
SQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCC
NDESGEDVEVPYVRYTIR*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with X56976 CDS: ; ; g569a (G190D), c1300g (Q434E)- these changes present in all E1a published sequences for all other species and in other human sequences (e.g. AAP36419, NP_695012) ; ; g1340a (R447H) – this change present in some mouse E1a sequences and in human EST sequences (e.g. IMAGE consortium EST clones 6537166, 6598339, 6464027).