cDNA Clone Ku80

• Plasmid: pEBG6P-1 Ku80
• Parent Plasmid: pEBG6P
• Expressed: GST-Ku80
• Genbank: J04977
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGTGCGGTCGGGGAATAAGGCAGCTGTTGTGCTGTGTATGGACGTGGGCTTTACCATGAGTAACTCCATTCCTGGTATAGAATCCCCATTTGAACAAGCAAAGAAGGTGATAACCATGTTTGTACAGCGACAGGTGTTTGCTGAGAACAAGGATGAGATTGCTTTAGTCCTGTTTGGTACAGATGGCACTGACAATCCCCTTTCTGGTGGGGATCAGTATCAGAACATCACAGTGCACAGACATCTGATGCTACCAGATTTTGATTTGCTGGAGGACATTGAAAGCAAAATCCAACCA
GGTTCTCAACAGGCTGACTTCCTGGATGCACTAATCGTGAGCATGGATGT
GATTCAACATGAAACAATAGGAAAGAAGTTTGAGAAGAGGCATATTGAAA
TATTCACTGACCTCAGCAGCCGATTCAGCAAAAGTCAGCTGGATATTATA
ATTCATAGCTTGAAGAAATGTGACATCTCCCTGCAATTCTTCTTGCCTTT
CTCACTTGGCAAGGAAGATGGAAGTGGGGACAGAGGAGATGGCCCCTTTC
GCTTAGGTGGCCATGGGCCTTCCTTTCCACTAAAAGGAATTACCGAACAG
CAAAAAGAAGGTCTTGAGATAGTGAAAATGGTGATGATATCTTTAGAAGG
TGAAGATGGGTTGGATGAAATTTATTCATTCAGTGAGAGTCTGAGAAAAC
TGTGCGTCTTCAAGAAAATTGAGAGGCATTCCATTCACTGGCCCTGCCGA
CTGACCATTGGCTCCAATTTGTCTATAAGGATTGCAGCCTATAAATCGAT
TCTACAGGAGAGAGTTAAAAAGACTTGGACAGTTGTGGATGCAAAAACCC
TAAAAAAAGAAGATATACAAAAAGAAACAGTTTATTGCTTAAATGATGAT
GATGAAACTGAAGTTTTAAAAGAGGATATTATTCAAGGGTTCCGCTATGG
AAGTGATATAGTTCCTTTCTCTAAAGTGGATGAGGAACAAATGAAATATA
AATCGGAGGGGAAGTGCTTCTCTGTTTTGGGATTTTGTAAATCTTCTCAG
GTTCAGAGAAGATTCTTCATGGGAAATCAAGTTCTAAAGGTCTTTGCAGC
AAGAGATGATGAGGCAGCTGCAGTTGCACTTTCCTCCCTGATTCATGCTT
TGGATGACTTAGACATGGTGGCCATAGTTCGATATGCTTATGACAAAAGA
GCTAATCCTCAAGTCGGCGTGGCTTTTCCTCATATCAAGCATAACTATGA
GTGTTTAGTGTATGTGCAGCTGCCTTTCATGGAAGACTTGCGGCAATACA
TGTTTTCATCCTTGAAAAACAGTAAGAAATATGCTCCCACCGAGGCACAG
TTGAATGCTGTTGATGCTTTGATTGACTCCATGAGCTTGGCAAAGAAAGA
TGAGAAGACAGACACCCTTGAAGACTTGTTTCCAACCACCAAAATCCCAA
ATCCTCGATTTCAGAGATTATTTCAGTGTCTGCTGCACAGAGCTTTACAT
CCCCGGGAGCCTCTACCCCCAATTCAGCAGCATATTTGGAATATGCTGAA
TCCTCCCGCTGAGGTGACAACGAAAAGTCAGATTCCTCTCTCTAAAATAA
AGACCCTTTTTCCTCTGATTGAAGCCAAGAAAAAGGATCAAGTGACTGCT
CAGGAAATTTTCCAAGACAACCATGAAGATGGACCTACAGCTAAAAAATT
AAAGACTGAGCAAGGGGGAGCCCACTTCAGCGTCTCCAGTCTGGCTGAAG
GCAGTGTCACCTCTGTTGGAAGTGTGAATCCTGCTGAAAACTTCCGTGTT
CTAGTGAAACAGAAGAAGGCCAGCTTTGAGGAAGCGAGTAACCAGCTCAT
AAATCACATCGAACAGTTTTTGGATACTAATGAAACACCGTATTTTATGA
AGAGCATAGACTGCATCCGAGCCTTCCGGGAAGAAGCCATTAAGTTTTCA
GAAGAGCAGCGCTTTAACAACTTCCTGAAAGCCCTTCAAGAGAAAGTGGA
AATTAAACAATTAAATCATTTCTGGGAAATTGTTGTCCAGGATGGAATTA
CTCTGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAGGAA
GCCAAAAAGTTTCTGGCCCCCAAAGACAAACCAAGTGGAGACACAGCAGC
TGTATTTGAAGAAGGTGGTGATGTGGACGATTTATTGGACATGATATAGggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVRSGNKAAVVLC
MDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDG
TDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADFLDALIV
SMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQF
FLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMI
SLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAA
YKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQG
FRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLK
VFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIK
HNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSL
AKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIW
NMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPT
AKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEAS
NQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQ
EKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSG
DTAAVFEEGGDVDDLLDMI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5211
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Ku80 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 110068 Daltons