cDNA Clone Cyclin A

• Plasmid: pFastBac1 Cyclin A.6His
• Parent Plasmid: pFastbac1
• Expressed: Cyclin A-6His
• Genbank: NM_001237
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccGAAACCATGTTGGGCAACTCTGCGCCGGGGCCTGCGACCCGCGAGGCGGGCTCGGCGCTGCTAGCATTGCAGCAGACGGCGCTCCAAGAGGACCAGGAGAATATCAACCCGGAAAAGGCAGCGCCCGTCCAACAACCGCGGACCCGGGCCGCGCTGGCGGTACTGAAGTCCGGGAACCCGCGGGGTCTAGCGCAGCAGCAGAGGCCGAAGACGAGACGGGTTGCACCCCTTAAGGATCTTCCTGTAAATGATGAGCATGTCACCGTTCCTCCTTGGAAAGCAAACAGTAAACAGCCTGCGTTCACCATTCATGTGGATGAAGCAGAAAAAGAAGCTCAGAAGAAGCCAGCTGAATCTCAAAAAATAGAGCGTGAAGATGCCCTGGCTTTTAATTCAGCCATTAGTTTACCTGGACCCAGAAAACCATTGGTCCCTCTTGATTATCCAATGGATGGTAGTTTTGAGTCACCACATACTATGGACATGTCAATTGTATTAGAAGATGAAAAGCCAGTGAGTGTTAATGAAGTACCAGACTACCATGAGGATATTCACACATACCTTAGGGAAATGGAGGTTAAATGTAAACCTAAAGTGGGTTACATG
AAGAAACAGCCAGACATCACTAACAGTATGAGAGCTATCCTCGTGGACTG
GTTAGTTGAAGTAGGAGAAGAATATAAACTACAAAATGAGACCCTGCATT
TGGCTGTGAACTACATTGATAGGTTCCTGTCTTCCATGTCAGTGCTGAGA
GGAAAACTTCAGCTTGTGGGCACTGCTGCTATGCTGTTAGCCTCAAAGTT
TGAAGAAATATACCCCCCAGAAGTAGCAGAGTTTGTGTACATTACAGATG
ATACCTACACCAAGAAACAAGTTCTGAGAATGGAGCATCTAGTTTTGAAA
GTCCTTACTTTTGACTTAGCTGCTCCAACAGTAAATCAGTTTCTTACCCA
ATACTTTCTGCATCAGCAGCCTGCAAACTGCAAAGTTGAAAGTTTAGCAA
TGTTTTTGGGAGAATTAAGTTTGATAGATGCTGACCCATACCTCAAGTAT
TTGCCATCAGTTATTGCTGGAGCTGCCTTTCATTTAGCACTCTACACAGT
CACGGGACAAAGCTGGCCTGAATCATTAATACGAAAGACTGGATATACCC
TGGAAAGTCTTAAGCCTTGTCTCATGGACCTTCACCAGACCTACCTCAAA
GCACCACAGCATGCACAACAGTCAATAAGAGAAAAGTACAAAAATTCAAA
GTATCATGGTGTTTCTCTCCTCAACCCACCAGAGACACTAAATCTGCACCATCACCATCACCATTAAgaattc

Amino Acid Sequence

MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAAL
AVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFT
IHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDG
SFESPHTMDMSIVLEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYM
KKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLR
GKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLK
VLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKY
LPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLK
APQHAQQSIREKYKNSKYHGVSLLNPPETLNLHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5209
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid for expression of full length cyclin A with C-terminal 6His tag.; N.B. Sequence difference compared with NM_001237 CDS:; G684a; silent in Q228.