cDNA Clone Ku70

• Plasmid: pEBG6P-1 Ku70
• Parent Plasmid: pEBG6P
• Expressed: GST-Ku70
• Genbank: J04611
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTCAGGGTGGGAGTCATATTACAAAACCGAGGGCGATGAAGAAGCAGAGGAAGAACAAGAAGAGAACCTTGAAGCAAGTGGAGACTATAAATATTCAGGAAGAGATAGTTTGATTTTTTTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCAGAGTGAAGATGAGTTGACACCTTTTGACATGAGCATCCAGTGTATCCAAAGTGTGTACATCAGTAAGATCATAAGCAGTGATCGAGATCTCTTGGCTGTGGTGTTCTATGGCACCGAGAAAGACAAAAATTCAGTGAATTTTAAA
AATATTTACGTCTTACAGGAGCTGGATAATCCAGGTGCAAAACGAATTCT
AGAGCTTGACCAGTTTAAGGGGCAGCAGGGACAAAAACGTTTCCAAGACA
TGATGGGCCACGGATCTGACTACTCACTCAGTGAAGTGCTGTGGGTCTGT
GCCAACCTCTTTAGTGATGTCCAATTCAAGATGAGTCATAAGAGGATCAT
GCTGTTCACCAATGAAGACAACCCCCATGGCAATGACAGTGCCAAAGCCA
GCCGGGCCAGGACCAAAGCCGGTGATCTCCGAGATACAGGCATCTTCCTT
GACTTGATGCACCTGAAGAAACCTGGGGGCTTTGACATATCCTTGTTCTA
CAGAGATATCATCAGCATAGCAGAGGATGAGGACCTCAGGGTTCACTTTG
AGGAATCCAGCAAGCTAGAAGACCTGTTGCGGAAGGTTCGCGCCAAGGAG
ACCAGGAAGCGAGCACTCAGCAGGTTAAAGCTGAAGCTCAACAAAGATAT
AGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCTCTCAAGCCTC
CTCCAATAAAGCTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACC
CGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAA
GAGGTCTCAGATCTATGGGAGTCGTCAGATTATACTGGAGAAAGAGGAAA
CAGAAGAGCTAAAACGGTTTGATGATCCAGGTTTGATGCTCATGGGTTTC
AAGCCGTTGGTACTGCTGAAGAAACACCATTACCTGAGGCCCTCCCTGTT
CGTGTACCCAGAGGAGTCGCTGGTGATTGGGAGCTCAACCCTGTTCAGTG
CTCTGCTCATCAAGTGTCTGGAGAAGGAGGTTGCAGCATTGTGCAGATAC
ACACCCCGCAGGAACATCCCTCCTTATTTTGTGGCTTTGGTGCCACAGGA
AGAAGAGTTGGATGACCAGAAAATTCAGGTGACTCCTCCAGGCTTCCAGC
TGGTCTTTTTACCCTTTGCTGATGATAAAAGGAAGATGCCCTTTACTGAA
AAAATCATGGCAACTCCAGAGCAGGTGGGCAAGATGAAGGCTATCGTTGA
GAAGCTTCGCTTCACATACAGAAGTGACAGCTTTGAGAACCCCGTGCTGC
AGCAGCACTTCAGGAACCTGGAGGCCTTGGCCTTGGATTTGATGGAGCCG
GAACAAGCAGTGGACCTGACATTGCCCAAGGTTGAAGCAATGAATAAAAG
ACTGGGCTCCTTGGTGGATGAGTTTAAGGAGCTTGTTTACCCACCAGATT
ACAATCCTGAAGGGAAAGTTACCAAGAGAAAACACGATAATGAAGGTTCT
GGAAGCAAAAGGCCCAAGGTGGAGTATTCAGAAGAGGAGCTGAAGACCCA
CATCAGCAAGGGTACGCTGGGCAAGTTCACTGTGCCCATGCTGAAAGAGG
CCTGCCGGGCTTACGGGCTGAAGAGTGGTCTGAAGAAGCAGGAGCTGCTG
GAAGCCCTCACCAAGCACTTCCAGGACTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSGWESYYKTEGD
EEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMS
IQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGA
KRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSH
KRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDI
SLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKL
NKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLP
SDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLR
PSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVAL
VPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMK
AIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEA
MNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEE
LKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5200
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Ku70 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 97215 Daltons