cDNA Clone CDK2

• Plasmid: pFBDual GST-CDK2/Cyclin A 171-432(end)
• Parent Plasmid: pFastbacDual
• Expressed: GST-CDK2/Cyclin A 171-432(end)
• Genbank: NM_001798 NM_001237
• Tag: GST

Sequence of Insert

DNA sequence of insert 1 (GST-CDK2) :
ctcgagGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGAATGGAGAACT
TCCAAAAGGTGGAAAAGATCGGAGAGGGCACGTACGGAGTTGTGTACAAA
GCCAGAAACAAGTTGACGGGAGAGGTGGTGGCGCTTAAGAAAATCCGCCT
GGACACTGAGACTGAGGGTGTGCCCAGTACTGCCATCCGAGAGATCTCTC
TGCTTAAGGAGCTTAACCATCCTAATATTGTCAAGCTGCTGGATGTCATT
CACACAGAAAATAAACTCTACCTGGTTTTTGAATTTCTGCACCAAGATCT
CAAGAAATTCATGGATGCCTCTGCTCTCACTGGCATTCCTCTTCCCCTCA
TCAAGAGCTATCTGTTCCAGCTGCTCCAGGGCCTAGCTTTCTGCCATTCT
CATCGGGTCCTCCACCGAGACCTTAAACCTCAGAATCTGCTTATTAACAC
AGAGGGGGCCATCAAGCTAGCAGACTTTGGACTAGCCAGAGCTTTTGGAG
TCCCTGTTCGTACTTACACCCATGAGGTGGTGACCCTGTGGTACCGAGCT
CCTGAAATCCTCCTGGGCTGCAAATATTATTCCACAGCTGTGGACATCTG
GAGCCTGGGCTGCATCTTTGCTGAGATGGTGACTCGCCGGGCCCTATTCC
CTGGAGATTCTGAGATTGACCAGCTCTTCCGGATCTTTCGGACTCTGGGG
ACCCCAGATGAGGTGGTGTGGCCAGGAGTTACTTCTATGCCTGATTACAA
GCCAAGTTTCCCCAAGTGGGCCCGGCAAGATTTTAGTAAAGTTGTACCTC
CCCTGGATGAAGATGGACGGAGCTTGTTATCGCAAATGCTGCACTACGAC
CCTAACAAGCGGATTTCGGCCAAGGCAGCCCTGGCTCACCCTTTCTTCCA
GGATGTGACCAAGCCAGTACCCCATCTTCGACTCTGActcgag

DNA sequence of insert 2 (Cyclin A 171-432) :
ggatccGAAACCATGAGTGTTAATGAAGTACCAGACTACCATGAGGATATTCACACATACCTTAGGGAAATGGAGGTTAAATGTAAACCTAAAGTGGGTTACATGAAGAAACAGCCAGACATCACTAACAGTATGAGAGCTATCCTCGTGGACTGGTTAGTTGAAGTAGGAGAAGAATATAAACTACAGAATGAGACCCTGCATTTGGCTGTGAACTACATTGATAGGTTCCTGTCTTCCATGTCAGTGCTGAGAGGAAAACTTCAGCTTGTGGGCACTGCTGCTATGCTGTTAGCCTCAAAGTTTGAAGAAATATACCCCCCAGAAGTAGCAGAGTTTGTGTACATTACAGATGATACCTACACCAAGAAACAAGTTCTGAGAATGGAGCATCTAGTTTTGAAAGTCCTTACTTTTGACTTAGCTGCTCCAACAGTAAATCAGTTTCTTACCCAATACTTTCTGCATCAGCAGCCTGCAAACTGCAAAGTTGAAAGTTTAGCAATGTTTTTGGGAGAATTAAGTTTGATAGATGCTGACCCATACCTCAAGTATTTGCCATCAGTTATTGCTGGAGCTGCCTTTCATTTAGCACTCTACACAGTCACGGGACAAAGCTGGCCTGAATCATTAATACGAAAGACTGGATATACCCTGGAAAGTCTTAAGCCTTGTCTCATGGACCTTCACCAGACCTACCTCAAAGCACCACAGCATGCACAACAGTCAATAAGAGAAAAGTACAAAAATTCAAAGTATCATGGT
GTTTCTCTCCTCAACCCACCAGAGACACTAAATCTGTAAgaattc

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (GST-CDK2) (p10 promoter):
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGMENFQKVEKIGEGTYGVVYK
ARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVI
HTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHS
HRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRA
PEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLG
TPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYD
PNKRISAKAALAHPFFQDVTKPVPHLRL*

Amino acid sequence of expressed protein 2 (Cyclin A 171-432) (Polyhedrin promoter) :

MSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLV
EVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEE
IYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYF
LHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTG
QSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYH
GVSLLNPPETLNL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDual GST-CDK2/Cyclin A 171-432(end)74.15 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU5189
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express N-terminally tagged CDK2 (with PreScission Protease site) and untagged Cyclin A 171-432(end).
• Low Temp: No