cDNA Clone CLK1

• Plasmid: pEBG6P-1 CLK1
• Parent Plasmid: pEBG6P
• Expressed: GST-CLK1
• Genbank: BC031549
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAGAACAAGCGCTGCAAATACAATCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAGATTATCATAGTCGACGCTACATTGATGAGTACAGAAATGACTACACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATGAAAGCCGGTATCAGAACCAT
AGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACA
CAGGATTCACCACAGTACTTCACATCGTCGTTCACATGGGAAGAGTCACC
GAAGGAAAAGAACCAGGAGTGTAGAGGATGATGAGGAGGGTCACCTGATC
TGTCAGAGTGGAGACGTACTAAGTGCAAGATATGAAATTGTTGATACTTT
AGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGATCATAAAGCGG
GAGGTAGACATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGT
GAAGCTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATACAACAGA
CCCCAACAGTACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATC
ATGGTCACATTTGCATTGTTTTTGAACTATTGGGACTTAGTACTTACGAC
TTCATTAAAGAAAATGGTTTTCTACCATTTCGACTGGATCATATCAGAAA
GATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAATAAGT
TGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGAC
TACACAGAGGCGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAAT
AAATCCAGATATTAAAGTTGTAGACTTTGGTAGTGCAACATATGATGACG
AACATCACAGTACATTGGTATCTACAAGACATTATAGAGCACCTGAAGTT
ATTTTAGCCCTAGGGTGGTCCCAACCATGTGATGTCTGGAGCATAGGATG
CATTCTTATTGAATACTATCTTGGGTTTACCGTATTTCCAACACACGATA
GTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAAA
CATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATT
AGACTGGGATGAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTA
AACCTCTGAAGGAATTTATGCTTTCTCAAGATGTTGAACATGAGCGTCTC
TTTGACCTCATTCAGAAAATGTTGGAGTATGATCCAGCCAAAAGAATTAC
TCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTGAAGAAAAGTA
TATAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5179
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 85016 Daltons