cDNA Clone PIM3

• Plasmid: pFB1 GST-6His-thrombin-PIM3
• Parent Plasmid: pFastbac1
• Expressed: GST-6His-thrombin-PIM3
• Genbank: Q86V86
• Tag: GST

Sequence of Insert

gaattcGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCACCATCACCATCACCATCTGGTTCCGCGTGGAATGCTGC
TCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCGGCGGCGTGGAC
CACCTCCCGGTGAAGATCCTGCAGCCAGCCAAGGCGGACAAGGAGAGCTT
CGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGGTAGCGGCGGCTTCGGCA
CGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGGTGGCTGTGAAG
CACGTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTGGGCGGCGCGAC
CGTGCCCCTGGAGGTGGTGCTGCTGCGCAAGGTGGGCGCGGCGGGCGGCG
CGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGCCCGACGGCTTC
CTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTCTTCGACTTTAT
CACGGAGCGCGGCGCCCTGGACGAGCCGCTGGCGCGCCGCTTCTTCGCGC
AGGTGCTGGCCGCCGTGCGCCACTGCCACAGCTGCGGGGTCGTGCACCGC
GACATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCCGGAGAGCTCAA
GCTCATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACACGGTCTACACCG
ACTTCGACGGCACCCGAGTGTACAGCCCCCCGGAGTGGATCCGCTACCAC
CGCTACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGCGTGCTTCTCTA
CGATATGGTGTGTGGGGACATCCCCTTCGAGCAGGACGAGGAGATCCTCC
GAGGCCGCCTGCTCTTCCGGAGGAGGGTCTCTCCAGAGTGCCAGCAGCTG
ATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAGCGGCCGTCGCTGGATCA
GATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGGCGCCCCGGAGA
GCTGTGACCTGCGGCTGTGCACCCTCGACCCTGATGACGTGGCCAGCACC
ACGTCCAGCAGCGAGAGCTTGTGAactagt

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDHHHHHHLVPRGMLLSKFGSLAHLCGPGGVD
HLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVK
HVVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGF
LLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHR
DIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYH
RYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQL
IRWCLSLRPSERPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVAST
TSSSESL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5175
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid. Thrombin cleavage site between 6His tag and PIM3.