cDNA Clone CAK1 (yeast)

• Synonyms: CDK7
• Plasmid: pFBHTb CAK1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-CAK1
• Genbank: U60192
• Tag: 6His
• Species: Yeast

Sequence of Insert

ggatccAAACTGGATAGTATAGACATTACACACTGTCAGTTGGTCAAATCTACTAGAACTGCTAGGATTTATAGGTCGGATACATATGCCATTAAATGTCTAGCACTAGATTTCGATATCCCGCCACATAACGCCAAATTCGAAGTATCGATA
TTAAACAAACTGGGCAACAAATGTAAGCACATCTTACCTCTTCTAGAGTC
TAAGGCTACCGATAATAATGACCTATTGTTGTTGTTTCCCTTTGAAGAGA
TGAACCTTTATGAGTTCATGCAAATGCACTATAAAAGAGATAGAAGAAAA
AAAAATCCCTATTACGATTTGCTAAATCCCAGTATCCCAATTGTTGCGGA
CCCCCCCGTTCAGAAATATACTAATCAATTGGACGTCAATCGGTATTCTT
TGTCCTTTTTCCGGCAAATGGTTGAAGGGATTGCATTCTTACATGAGAAC
AAGATCATTCACCGCGACATCAAACCGCAAAATATCATGCTAACAAACAA
TACCAGCACCGTATCCCCAAAGTTGTACATAATTGATTTTGGCATCTCTT
ATGACATGGCAAATAACTCACAAACAAGTGCGGAACCCATGGATAGCAAG
GTGACGGATATAAGCACAGGAATTTACAAGGCCCCAGAAGTGCTTTTTGG
AGTAAAATGCTATGATGGTGGCGTGGACGTGTGGTCGTTGTTGATAATTA
TTTCTCAGTGGTTCCAGAGAGAAACAAGCCGTATGGGGCACGTTCCGGCC
ATGATTGATGACGGCAGCGACGACATGAACTCAGATGGAAGCGATTTCAG
ACTGATTTGCTCAATATTCGAAAAGTTGGGCATACCGTCCATTCAGAAAT
GGGAAGAGGTTGCGCAACACGGCTCGGTTGATGCATTTGTTGGTATGTTT
GGTGCAGATGGCGATGGCAAGTATGTACTGGACCAGGAGAAAGATGTACA
GATTAGCATTGTTGAGAGGAATATGCCTCGACTGGACGAGATTGCGGATG
TCAAAGTCAAGCAGAAGTTCATTAATTGTATCCTGGGGATGGTTTCATTT
TCACCAAACGAAAGATGGAGCTGTCAAAGAATCTTGCAAGAATTAGAAAA
GCCATAAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSKLDSIDITHCQLVKSTRTARIY
RSDTYAIKCLALDFDIPPHNAKFEVSILNKLGNKCKHILPLLESKATDNN
DLLLLFPFEEMNLYEFMQMHYKRDRRKKNPYYDLLNPSIPIVADPPVQKY
TNQLDVNRYSLSFFRQMVEGIAFLHENKIIHRDIKPQNIMLTNNTSTVSP
KLYIIDFGISYDMANNSQTSAEPMDSKVTDISTGIYKAPEVLFGVKCYDG
GVDVWSLLIIISQWFQRETSRMGHVPAMIDDGSDDMNSDGSDFRLICSIF
EKLGIPSIQKWEEVAQHGSVDAFVGMFGADGDGKYVLDQEKDVQISIVER
NMPRLDEIADVKVKQKFINCILGMVSFSPNERWSCQRILQELEKP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5172
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 45397 Daltons