cDNA Clone CAK1 (yeast)

• Synonyms: CDK7
• Plasmid: pMalP2X CAK1.6His
• Parent Plasmid: pMALP2X
• Expressed: MBP CAK1-6His
• Genbank: U60192
• Tag: MBP
• Species: Yeast

Sequence of Insert

ggatccAAACTGGATAGTATAGACATTACACACTGTCAGTTGGTCAAATC
TACTAGAACTGCTAGGATTTATAGGTCGGATACATATGCCATTAAATGTC
TAGCACTAGATTTCGATATCCCGCCACATAACGCCAAATTCGAAGTATCG
ATATTAAACAAACTGGGCAACAAATGTAAGCACATCTTACCTCTTCTAGA
GTCTAAGGCTACCGATAATAATGACCTATTGTTGTTGTTTCCCTTTGAAG
AGATGAACCTTTATGAGTTCATGCAAATGCACTATAAAAGAGATAGAAGA
AAAAAAAATCCCTATTACGATTTGCTAAATCCCAGTATCCCAATTGTTGC
GGACCCCCCCGTTCAGAAATATACTAATCAATTGGACGTCAATCGGTATT
CTTTGTCCTTTTTCCGGCAAATGGTTGAAGGGATTGCATTCTTACATGAG
AACAAGATCATTCACCGCGACATCAAACCGCAAAATATCATGCTAACAAA
CAATACCAGCACCGTATCCCCAAAGTTGTACATAATTGATTTTGGCATCT
CTTATGACATGGCAAATAACTCACAAACAAGTGCGGAACCCATGGATAGC
AAGGTGACGGATATAAGCACAGGAATTTACAAGGCCCCAGAAGTGCTTTT
TGGAGTAAAATGCTATGATGGTGGCGTGGACGTGTGGTCGTTGTTGATAA
TTATTTCTCAGTGGTTCCAGAGAGAAACAAGCCGTATGGGGCACGTTCCG
GCCATGATTGATGACGGCAGCGACGACATGAACTCAGATGGAAGCGATTT
CAGACTGATTTGCTCAATATTCGAAAAGTTGGGCATACCGTCCATTCAGA
AATGGGAAGAGGTTGCGCAACACGGCTCGGTTGATGCATTTGTTGGTATG
TTTGGTGCAGATGGCGATGGCAAGTATGTACTGGACCAGGAGAAAGATGT
ACAGATTAGCATTGTTGAGAGGAATATGCCTCGACTGGACGAGATTGCGG
ATGTCAAAGTCAAGCAGAAGTTCATTAATTGTATCCTGGGGATGGTTTCA
TTTTCACCAAACGAAAGATGGAGCTGTCAAAGAATCTTGCAAGAATTAGA
AAAGCCAGGTTCACACCACCACCACCACCACTAAGAATTCCCGGgtcgac

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSKLDSIDITHCQLVKSTRTARIYRSDTYAIKCLALDFDIPPHNAKFEVSILNKLGNKCKHILPLLESKATDNNDLLLLFPFEEMNLYEFMQMHYKRDRRKKNPYYDLLNPSIPIVADPPVQKYTNQLDVNRYSLSFFRQMVEGIAFLHENKIIHRDIKPQNIMLTNNTSTVSPKLYIIDFGISYDMANNSQTSAEPMDSKVTDISTGIYKAPEVLFGVKCYDGGVDVWSLLIIISQWFQRETSRMGHVPAMIDDGSDDMNSDGSDFRLICSIFEKLGIPSIQKWEEVAQHGSVDAFVGMFGADGDGKYVLDQEKDVQISIVERNMPRLDEIADVKVKQKFINCILGMVSFSPNERWSCQRILQELEKPGSHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5140
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express MBP N terminal fusion.