cDNA Clone CDK4

• Plasmid: pFB.HTb.PP CDK4
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-CDK4
• Genbank: NM_000075
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATGGGACAGTGTACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGTGAGAGTCCCCAATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGTGGCTTTACTGAGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGTCTGTGCCACATCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGACCAGGACCTA
AGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGAT
CAAGGATCTGATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCA
ATTGCATCGTTCACCGAGATCTGAAGCCAGAGAACATTCTGGTGACAAGT
GGTGGAACAGTCAAGCTGGCTGACTTTGGCCTGGCCAGAATCTACAGCTA
CCAGATGGCACTTACACCCGTGGTTGTTACACTCTGGTACCGAGCTCCCG
AAGTTCTTCTGCAGTCCACATATGCAACACCTGTGGACATGTGGAGTGTT
GGCTGTATCTTTGCAGAGATGTTTCGTCGAAAGCCTCTCTTCTGTGGAAA
CTCTGAAGCCGACCAGTTGGGCAAAATCTTTGACCTGATTGGGCTGCCTC
CAGAGGATGACTGGCCTCGAGATGTATCCCTGCCCCGTGGAGCCTTTCCC
CCCAGAGGGCCCCGCCCAGTGCAGTCGGTGGTACCTGAGATGGAGGAGTC
GGGAGCACAGCTGCTGCTGGAAATGCTGACTTTTAACCCACACAAGCGAA
TCTCTGCCTTTCGAGCTCTGCAGCACTCTTATCTACATAAGGATGAAGGT
AATCCGGAGTGAggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSLEVLFQGPLGSMATSRYEPVAE
IGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLR
RLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPG
LPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGL
ARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRK
PLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVV
PEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag and PreScission protease site); ; Estimated MW of expressed protein : 38218 Daltons