cDNA Clone PKC alpha

• Plasmid: pGEX6P-1-PKC alpha
• Parent Plasmid: pGEX6P1
• Expressed: GST-PKC alpha
• Genbank: NM_002737
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGCTGACGTTTTCCCGGGCAACGACTCCACGGCGTCTCAGGACGTGGCCAACCGCTTCGCCCGCAAAGGGGCGCTGAGGCAGAAGAACGTGCACGAGGTGAAGGACCACAAATTCATCGCGCGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGGTTTGGGAAACAAGGCTTCCAGTGCCAAGTTTGCTGTTTTGTGGTCCACAAGAGGTGCCATGAATTTGTTACTTTTTCTTGTCCGGGTGCGGATAAGGGACCCGACACTGATGACCCCAGGAGC
AAGCACAAGTTCAAAATCCACACTTACGGAAGCCCCACCTTCTGCGATCA
CTGTGGGTCACTGCTCTATGGACTTATCCATCAAGGGATGAAATGTGACA
CCTGCGATATGAACGTTCACAAGCAATGCGTCATCAATGTCCCCAGCCTC
TGCGGAATGGATCACACTGAGAAGAGGGGGCGGATTTACCTAAAGGCTGA
GGTTGCTGATGAAAAGCTCCATGTCACAGTACGAGATGCAAAAAATCTAA
TCCCTATGGATCCAAACGGGCTTTCAGATCCTTATGTGAAGCTGAAACTT
ATTCCTGATCCCAAGAATGAAAGCAAGCAAAAAACCAAAACCATCCGCTC
CACACTAAATCCGCAGTGGAATGAGTCCTTTACATTCAAATTGAAACCTT
CAGACAAAGACCGACGACTGTCTGTAGAAATCTGGGACTGGGATCGAACA
ACAAGGAATGACTTCATGGGATCCCTTTCCTTTGGAGTTTCGGAGCTGAT
GAAGATGCCGGCCAGTGGATGGTACAAGTTGCTTAACCAAGAAGAAGGTG
AGTACTACAACGTACCCATTCCGGAAGGGGACGAGGAAGGAAACATGGAA
CTCAGGCAGAAATTCGAGAAAGCCAAACTTGGCCCTGCTGGCAACAAAGT
CATCAGTCCCTCTGAAGACAGGAAACAACCTTCCAACAACCTTGACCGAG
TGAAACTCACGGACTTCAATTTCCTCATGGTGTTGGGAAAGGGGAGTTTT
GGAAAGGTGATGCTTGCCGACAGGAAGGGCACAGAAGAACTGTATGCAAT
CAAAATCCTGAAGAAGGATGTGGTGATTCAGGATGATGACGTGGAGTGCA
CCATGGTAGAAAAGCGAGTCTTGGCCCTGCTTGACAAACCCCCGTTCTTG
ACGCAGCTGCACTCCTGCTTCCAGACAGTGGATCGGCTGTACTTCGTCAT
GGAATATGTCAACGGTGGGGACCTCATGTACCACATTCAGCAAGTAGGAA
AATTTAAGGAACCACAAGCAGTATTCTATGCGGCAGAGATTTCCATCGGA
TTGTTCTTTCTTCATAAAAGAGGAATCATTTATAGGGATCTGAAGTTAGA
TAACGTCATGTTGGATTCAGAAGGACATATCAAAATTGCTGACTTTGGGA
TGTGCAAGGAACACATGATGGATGGAGTCACGACCAGGACCTTCTGTGGG
ACTCCAGATTATATCGCCCCAGAGATAATCGCTTATCAGCCGTATGGAAA
ATCTGTGGACTGGTGGGCCTACGGCGTCCTGTTGTATGAAATGCTTGCCG
GGCAGCCTCCATTTGATGGTGAAGATGAAGACGAGCTATTTCAGTCTATC
ATGGAGCACAACGTTTCCTATCCAAAATCCTTGTCCAAGGAGGCTGTTTC
TATCTGCAAAGGACTGATGACCAAACACCCAGCCAAGCGGCTGGGCTGTG
GGCCTGAGGGGGAGAGGGACGTGAGAGAGCATGCCTTCTTCCGGAGGATC
GACTGGGAAAAACTGGAGAACAGGGAGATCCAGCCACCATTCAAGCCCAA
AGTGTGTGGCAAAGGAGCAGAGAACTTTGACAAGTTCTTCACACGAGGAC
AGCCCGTCTTAACACCACCTGATCAGCTGGTTATTGCTAACATAGACCAG
TCTGATTTTGAAGGGTTCTCGTATGTCAACCCCCAGTTTGTGCACCCCAT
CTTACAGAGTGCAGTATGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMADVFPGNDSTASQDV
ANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQ
CQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCD
HCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKA
EVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIR
STLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSEL
MKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKLGPAGNK
VISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYA
IKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFV
MEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKL
DNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYG
KSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAV
SICKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKP
KVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHP
ILQSAV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5120
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 103893 Daltons