cDNA Clone NUAK2

• Plasmid: pGEX6P-1-NUAK2 1-355 (kinase domain) (EcoR1/Not1)
• Parent Plasmid: pGEX6P1
• Expressed: GST-NUAK2 1-355 (kinase domain)
• Genbank: NM_030952
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAG
ATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGA
AGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCC
GGGGCGACCTTTATGACTACATCAGCGAGCGGCAGCAGCTCAGTGAGCGC
GAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGCACTATTGCCA
TCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGG
ATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTAC
CATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTC
GCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCT
GGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTT
GATGGGCATGACCATAAGATCCTAGTGAAACAGATCAGCAACGGGGCCTA
CCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATCCGGTGGCTGT
TGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCAC
TGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCC
GCATGAGGGTGGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTG
ACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTG
TGCAGCTTCTTCAAGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMESLVFARRSGPTPSA
AELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGK
VKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIH
EVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYC
HQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYA
SPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGA
YREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEA
PHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 67589 Daltons