cDNA Clone NUAK1

• Plasmid: pGEX6P-1-NUAK1 1-355 (kinase domain)
• Parent Plasmid: pGEX6P1
• Expressed: GST-NUAK1 1-355 (kinase domain)
• Genbank: NM_014840
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGAAGGGGCCGCCGCGCCTGTGGCGGGGGACCGCCCCGACTTGGGGCTGGGGGCGCCGGGCTCTCCCCGAGAGGCGGTGGCGGGGGCGACTGCAGCCCTGGAGCCCAGGAAGCCGCACGGGGTGAAGCGGCATCACCACAAGCACAACTTGAAGCACCGCTACGAGCTGCAGGAGACCCTGGGCAAAGGCACCTACGGCAAAGTCAAGCGGGCCACCGAGAGGTTTTCTGGCCGAGTGGTTGCTATAAAATCCATTCGTAAGGACAAAATTAAGGATGAACAAGACATGGTTCACATC
AGACGAGAGATTGAGATCATGTCATCTCTCAACCATCCTCATATCATCAG
TATTTATGAAGTGTTTGAGAACAAAGATAAGATTGTGATCATCATGGAAT
ATGCCAGCAAAGGGGAGCTGTACGATTACATCAGTGAGCGGCGACGCCTC
AGTGAGAGGGAGACCCGGCACTTCTTCCGGCAGATCGTCTCTGCTGTGCA
CTATTGTCACAAGAACGGTGTGGTCCACCGGGACTTGAAGCTGGAAAATA
TACTGCTCGATGACAACTGCAATATTAAGATTGCTGACTTTGGGCTTTCC
AACCTGTACCAGAAGGATAAGTTCTTACAAACGTTTTGTGGGAGTCCACT
CTATGCATCTCCTGAGATTGTCAATGGGAGACCTTACCGAGGGCCAGAGG
TGGACAGCTGGGCCCTGGGTGTGTTGCTTTACACTCTTGTTTATGGAACA
ATGCCCTTCGATGGTTTCGATCACAAAAACCTCATTCGGCAAATCAGCAG
CGGAGAGTACCGGGAGCCAACACAGCCCTCAGATGCTCGAGGACTCATAC
GGTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATTGAGGACATT
GCCAACCACTGGTGGGTGAACTGGGGCTATAAGAGCAGCGTGTGTGACTG
TGATGCCCTCCATGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACT
GGCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCCAAAATGAAG
GGCCTGGCCAAACCCTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMEGAAAPVAGDRPDLG
LGAPGSPREAVAGATAALEPRKPHGVKRHHHKHNLKHRYELQETLGKGTY
GKVKRATERFSGRVVAIKSIRKDKIKDEQDMVHIRREIEIMSSLNHPHII
SIYEVFENKDKIVIIMEYASKGELYDYISERRRLSERETRHFFRQIVSAV
HYCHKNGVVHRDLKLENILLDDNCNIKIADFGLSNLYQKDKFLQTFCGSP
LYASPEIVNGRPYRGPEVDSWALGVLLYTLVYGTMPFDGFDHKNLIRQIS
SGEYREPTQPSDARGLIRWMLMVNPDRRATIEDIANHWWVNWGYKSSVCD
CDALHDSESPLLARIIDWHHRSTGLQADTEAKMKGLAKP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5102
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion. ; Estimated MW of expressed protein : 67712 Daltons