cDNA Clone BRSK1

• Plasmid: pGEX6P-1-BRSK1 1-400 (kinase domain)
• Parent Plasmid: pGEX6P1
• Expressed: GST-BRSK1 1-400 (kinase domain)
• Genbank: NM_032430
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTA
CCACCTCCCCCACCCCCACCCCCACCCACCCCAGCACGCCCAATATGTGG
GCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGGCTGGTT
AAACTCGGGGTCCACTGCATCACGGGTCAGAAGGTCGCCATCAAGATCGT
GAACCGGGAGAAGCTGTCGGAGTCGGTGCTGATGAAGGTGGAGCGGGAGA
TCGCCATCCTGAAGCTCATCGAACACCCACATGTCCTCAAGCTCCACGAC
GTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGG
GGGTGAGCTATTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGG
AGGCCCGAAAGTTCTTCCGCCAGATTGTGTCTGCGCTGGACTTCTGCCAC
AGCTACTCCATCTGCCACAGAGACCTAAAGCCCGAGAACCTGCTTTTGGA
TGAGAAAAACAACATCCGCATTGCAGACTTCGGCATGGCGTCCCTGCAGG
TGGGGGACAGCCTCCTGGAGACCAGCTGCGGGTCCCCCCATTATGCGTGT
CCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGCCGGGCAGACATGTG
GAGCTGTGGAGTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTG
ATGACGACAACCTCCGCCAGCTGCTGGAGAAGGTGAAACGGGGCGTCTTC
CACATGCCCCACTTCATTCCTCCAGATTGCCAGAGCCTCCTGAGGGGAAT
GATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAAATTCAGAAAC
ATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCA
GCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCCATCCAACGGAGAGCT
GGACCCCGACGTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAGGGACC
GCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAG
ATGATATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGA
GGACCAGGACCTGCCTCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTG
TGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCGGAAG
TCCATGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSSGAKEGGGGSPAYHLPH
PHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREK
LSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELF
DYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNN
IRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGV
ILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVE
PEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDV
LESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDL
PPRNDVDPPRKRVDSPMLSRHGKRRPERKSM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5100
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 72512 Daltons