cDNA Clone PIM3 (mouse)

• Plasmid: pEGFP-C3-PIM3 mRNA.FLAG
• Parent Plasmid: pEGFPC3
• Genbank: NM_145478
• Tag: GFP
• Species: Mouse

Sequence of Insert

gaattcGAGCGTCCGGCTTCCCCAGCACCGCCCTGCGAGACTCAAAACAGCCACACCGCAAAGCGAGCCTCGGGCGGAAGGAGGCGGAGCTTCAGGCGGCCCCGCCTCCGCGGAAGGATACACATCTCCGTGGTCCAAAACCCCGGGGCGAGGCGGCCGGGGCGTGTGAGCTGCTCGGCCAGCTGCCGTCTACGCGCTTTCGCGCGGCCACCGGGCAACTGCGCCGCGCGGCTGCCCCGCTGAGCGCTCGGCCTCGGGGCCGTGGGATCCGCCGCGCTGTCTGCGGTCAGGAAGACCGCCCTCCCG
CGTCCTTGCCGGACGGGTCAGAGGCGGCACCGCACGCGAGGCCACCCGCG
ATGCTGCTGTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCTGGCGG
CGTGGACCACCTCCCAGTGAAGATCCTACAGCCAGCCAAGGCTGACAAGG
AGAGCTTCGAGAAGGTGTACCAGGTGGGCGCCGTGCTGGGCAGCGGCGGC
TTCGGCACGGTCTACGCGGGCAGCCGCATCGCCGACGGACTCCCGGTGGC
TGTGAAGCACGTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGTCTCGGCG
GAGTGGCCGTGCCCCTGGAGGTGGTGCTGCTGCGCAAGGTGGGCGCGGCG
GGCGGCGCGCGCGGCGTCATCCGCTTGCTGGACTGGTTCGAGCGGCCCGA
CGGCTTCTTGTTGGTGCTGGAGCGACCCGAGCCGGCACAGGACCTCTTCG
ACTTCATCACTGAACGAGGCGCCCTGGACGAGCCGCTGGCGCGTCGCTTC
TTCGCGCAGGTGCTTGCCGCTGTGCGGCACTGCCACAATTGTGGGGTCGT
GCACCGCGACATCAAGGACGAGAACCTGCTGGTGGACCTGCGCTCGGGAG
AGCTGAAGCTCATCGACTTCGGCTCGGGCGCGGTGCTCAAGGACACGGTC
TACACTGACTTTGATGGCACCCGTGTGTACAGCCCCCCAGAGTGGATCCG
ATATCACCGATATCACGGGCGGTCTGCCACTGTGTGGTCTCTGGGTGTAC
TGCTCTACGACATGGTGTGTGGGGACATTCCCTTTGAGCAGGATGAGGAG
ATCTTGCGCGGCAGGCTCTTTTTCCGGAGGAGGGTCTCCCCAGAGTGCCA
GCAGCTTATTGAGTGGTGTCTCTCCCTGAGGCCCTCAGAGAGGCCCTCCC
TGGACCAAATTGCTGCCCACCCCTGGATGCTGGGGACAGAGGGGAGCGTT
CCAGAGAACTGTGACCTTCGGCTTTGTGCCCTGGATACTGACGACGGAGC
CAGTACCACTTCCAGCAGTGAGAGCTTGGACTACAAGGACGACGATGACAAGTGAgaattc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKYSDLELKLRIR
ASGFPSTALRDSKQPHRKASLGRKEAELQAAPPPRKDTHLRGPKPRGEAA
GACELLGQLPSTRFRAATGQLRRAAAPLSARPRGRGIRRAVCGQEDRPPA
SLPDGSEAAPHARPPAMLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKE
SFEKVYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGG
VAVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFD
FITERGALDEPLARRFFAQVLAAVRHCHNCGVVHRDIKDENLLVDLRSGE
LKLIDFGSGAVLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVL
LYDMVCGDIPFEQDEEILRGRLFFRRRVSPECQQLIEWCLSLRPSERPSL
DQIAAHPWMLGTEGSVPENCDLRLCALDTDDGASTTSSSESLDYKDDDDK
*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5087
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct to express GFP N-terminal fusion.