cDNA Clone PKC alpha

• Plasmid: pFastBacHTa PKC alpha
• Parent Plasmid: pSC-A
• Expressed: 6His-PKC alpha
• Genbank: NM_002737
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcATGGCTGACGTTTTCCCGGGCAACGACTCCACGGCGTCTCAGGACGTGGCCAACCGCTTCGCCCGCAAAGGGGCGCTGAGGCAGAAGAACGTGCACGAGGTGAAGGACCACAAATTCATCGCGCGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGGTTTGGGAAACAAGGCTTCCAGTGCCAAGTTTGCTGTTTTGTGGTCCACAAGAGGTGCCATGAATTTGTTACTTTTTCTTGTCCGGGTGCGGATAAGGGACCCGACACTGATGACCCCAGGAGC
AAGCACAAGTTCAAAATCCACACTTACGGAAGCCCCACCTTCTGCGATCA
CTGTGGGTCACTGCTCTATGGACTTATCCATCAAGGGATGAAATGTGACA
CCTGCGATATGAACGTTCACAAGCAATGCGTCATCAATGTCCCCAGCCTC
TGCGGAATGGATCACACTGAGAAGAGGGGGCGGATTTACCTAAAGGCTGA
GGTTGCTGATGAAAAGCTCCATGTCACAGTACGAGATGCAAAAAATCTAA
TCCCTATGGATCCAAACGGGCTTTCAGATCCTTATGTGAAGCTGAAACTT
ATTCCTGATCCCAAGAATGAAAGCAAGCAAAAAACCAAAACCATCCGCTC
CACACTAAATCCGCAGTGGAATGAGTCCTTTACATTCAAATTGAAACCTT
CAGACAAAGACCGACGACTGTCTGTAGAAATCTGGGACTGGGATCGAACA
ACAAGGAATGACTTCATGGGATCCCTTTCCTTTGGAGTTTCGGAGCTGAT
GAAGATGCCGGCCAGTGGATGGTACAAGTTGCTTAACCAAGAAGAAGGTG
AGTACTACAACGTACCCATTCCGGAAGGGGACGAGGAAGGAAACATGGAA
CTCAGGCAGAAATTCGAGAAAGCCAAACTTGGCCCTGCTGGCAACAAAGT
CATCAGTCCCTCTGAAGACAGGAAACAACCTTCCAACAACCTTGACCGAG
TGAAACTCACGGACTTCAATTTCCTCATGGTGTTGGGAAAGGGGAGTTTT
GGAAAGGTGATGCTTGCCGACAGGAAGGGCACAGAAGAACTGTATGCAAT
CAAAATCCTGAAGAAGGATGTGGTGATTCAGGATGATGACGTGGAGTGCA
CCATGGTAGAAAAGCGAGTCTTGGCCCTGCTTGACAAACCCCCGTTCTTG
ACGCAGCTGCACTCCTGCTTCCAGACAGTGGATCGGCTGTACTTCGTCAT
GGAATATGTCAACGGTGGGGACCTCATGTACCACATTCAGCAAGTAGGAA
AATTTAAGGAACCACAAGCAGTATTCTATGCGGCAGAGATTTCCATCGGA
TTGTTCTTTCTTCATAAAAGAGGAATCATTTATAGGGATCTGAAGTTAGA
TAACGTCATGTTGGATTCAGAAGGACATATCAAAATTGCTGACTTTGGGA
TGTGCAAGGAACACATGATGGATGGAGTCACGACCAGGACCTTCTGTGGG
ACTCCAGATTATATCGCCCCAGAGATAATCGCTTATCAGCCGTATGGAAA
ATCTGTGGACTGGTGGGCCTACGGCGTCCTGTTGTATGAAATGCTTGCCG
GGCAGCCTCCATTTGATGGTGAAGATGAAGACGAGCTATTTCAGTCTATC
ATGGAGCACAACGTTTCCTATCCAAAATCCTTGTCCAAGGAGGCTGTTTC
TATCTGCAAAGGACTGATGACCAAACACCCAGCCAAGCGGCTGGGCTGTG
GGCCTGAGGGGGAGAGGGACGTGAGAGAGCATGCCTTCTTCCGGAGGATC
GACTGGGAAAAACTGGAGAACAGGGAGATCCAGCCACCATTCAAGCCCAA
AGTGTGTGGCAAAGGAGCAGAGAACTTTGACAAGTTCTTCACACGAGGAC
AGCCCGTCTTAACACCACCTGATCAGCTGGTTATTGCTAACATAGACCAG
TCTGATTTTGAAGGGTTCTCGTATGTCAACCCCCAGTTTGTGCACCCCAT
CTTACAGAGTGCAGTATGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMADVFPGNDSTASQDVANRF
ARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVC
CFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGS
LLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVAD
EKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLN
PQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMP
ASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKLGPAGNKVISP
SEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKIL
KKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYV
NGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVM
LDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVD
WWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICK
GLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCG
KGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQS
AV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5084
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; N.B. sequence difference compared with NM_002737: t1572c (silent in Y524); Estimated MW of expressed protein : 80427 Daltons