cDNA Clone EWS

• Plasmid: pEBG6P-1 EWS T79A ETV1
• Parent Plasmid: pEBG6P
• Expressed: GST-EWS T79A ETV1
• Genbank: NM_005243 for EWS. NM_004956 for ETV1.
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTGCTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGAGACATCAAACAAGAGCCAGGAATGTATCGGGAAGGACCCACATACCAACGGCGAGGATCACTTCAGCTCTGGCAGTTTTTGGTAGCTCTTCTGGATGACCCTTCAAATTCTCATTTTATTGCCTGGACTGGTCGAGGCATGGAATTTAAACTGATTGAGCCTGAAGAGGTGGCCCGACGTTGGGGCATTCAGAAAAACAGGCCAGCTATGAACTATGATAAACTTAGCCGTTCACTCCGCTATTACTATGAGAAAGGAATTATGCAAAAGGTGGCTGGAGAGAGATATGTCTACAAGTTTGTGTGTGATCCAGAAGCCCTTTTCTCCATGGCCTTTCCAGATAATCAGCGTCCACTGCTGAAGACAGACATGGAACGTCACATCAACGAGGAGGACACAGTGCCTCTTTCTCACTTTGATGAGAGCATGGCCTACATGCCGGAAGGGGGCTGCTGCAACCCCCACCCCTACAACGAAGGCTACGTGTATTAAgtcgacgcaagggcgaattctgcagatatccatcacactggcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAASTD
YSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQA
QTTATYGQTAYATSYGQPPTGYTAPTAPQAYSQPVQGYGTGAYDTTTATV
TTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSST
GGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQ
SSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYS
QQSSSYGQQRDIKQEPGMYREGPTYQRRGSLQLWQFLVALLDDPSNSHFI
AWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQK
VAGERYVYKFVCDPEALFSMAFPDNQRPLLKTDMERHINEEDTVPLSHFD
ESMAYMPEGGCCNPHPYNEGYVY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5080
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express EWS T79A-ETV1 with N-terminal GST (contains precission protease site); Estimated MW of expressed protein : 75377