cDNA Clone EWS

• Plasmid: pEBG6P-1 EWS T79A
• Parent Plasmid: pEBG6P
• Expressed: GST-EWS T79A
• Genbank: NM_005243
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcGATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTGCTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAA
CCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACA
GAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAG
TCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCG
ACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACC
GAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC
AGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCT
CCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATT
CCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAGGAGTCTGGAG
GATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGG
GGCAGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCG
GGGAGGAGGACGCGGTGGAATGGGCAGCGCTGGAGAGCGAGGTGGCTTCA
ATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCA
CCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGG
ATTAAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGCAGT
GTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCATGATCCACATC
TACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTA
TGAAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAG
ATTTTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGCCTCCA
ATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCC
ACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCA
TGGGTCGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCAAGA
GGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTCCAGCA
CCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACT
TCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGC
TTCCTCCCGCCACCCTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGGTGG
CCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTC
CCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGT
GGTGGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGGTGG
CCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGAGGAAGAAGAG
GAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAG
CGCAGAGATCGGCCCTACTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAST
DYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQ
AQTTATYGQTAYATSYGQPPTGYTAPTAPQAYSQPVQGYGTGAYDTTTAT
VTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSS
TGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYD
QSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQY
SQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGR
GGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDP
DEDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDK
ETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSM
RGGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRG
SRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPP
PFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRG
MDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDR
PY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5073
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express EWS T79A with N-terminal GST (contains precission protease site); N.B. Sequence difference compared with NM_005243 (NP_005234): ; t1050a, silent in P350 and present in EST clones.; Estimated MW of expressed protein : 96609 Daltons