cDNA Clone NUCB1

• Plasmid: pEBG6P-1 NUCB1
• Parent Plasmid: pEBG6P
• Expressed: GST-NUCB1
• Genbank: NM_006184
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcGCCTCCCTCTGGGCCCCGAGGGACCCTCCTTCTGTTGCCGCT
GCTGCTGCTGCTCCTGCTTCGCGCCGTGCTGGCTGTCCCCCTGGAGCGAG
GGGCGCCCAACAAGGAGGAGACCCCTGCGACTGAGAGTCCCGACACAGGC
CTGTACTACCACCGGTACCTCCAGGAGGTCATCGATGTACTGGAGACGGA
TGGGCATTTCCGAGAGAAGCTGCAGGCTGCCAATGCGGAGGACATCAAGA
GCGGGAAGCTGAGCCGAGAGCTGGACTTTGTCAGCCACCACGTCCGCACC
AAGCTGGATGAGCTCAAGCGACAGGAGGTGTCACGGCTGCGGATGCTGCT
CAAGGCCAAGATGGACGCCGAGCAGGATCCCAATGTACAGGTGGATCATC
TGAATCTCCTGAAACAGTTTGAACACCTGGACCCTCAGAACCAGCATACA
TTCGAGGCCCGCGACCTGGAGCTGCTGATCCAGACGGCCACCCGGGACCT
TGCCCAGTACGACGCAGCCCATCATGAAGAGTTCAAGCGCTACGAGATGC
TTAAGGAACACGAGAGACGGCGTTATCTGGAGTCACTGGGAGAGGAGCAG
AGAAAGGAGGCGGAGAGGAAGCTGGAAGAGCAACAGCGCCGGCACCGCGA
GCACCCTAAAGTCAACGTGCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGT
GGGAGGAGCTGGATGGACTGGACCCCAACAGGTTTAACCCCAAGACCTTC
TTCATACCGCATGATATCAACAGTGATGGTGTCCTGGATGAGCAGGAGCT
GGAGGCACTCTTCACCAAGGAGCTGGAGAAAGTGTACGACCCGAAGAATG
AGGAGGACGACATGCGGGAGATGGAGGAGGAGCGACTGCGCATGCGGGAG
CATGTGATGAAGAATGTGGACACCAACCAGGACCGCCTCGTGACCCTGGA
GGAGTTCCTCGCATCCACTCAGAGGAAGGAGTTTGGGGACACCGGGGAGG
GCTGGGAGACAGTGGAGATGCACCCTGCCTACACCGAGGAAGAGCTGAGG
CGCTTTGAAGAGGAGCTGGCTGCCCGGGAGGCAGAGCTGAATGCCAAGGC
CCAGCGCCTCAGCCAGGAGACAGAGGCTCTAGGGCGGTCCCAGGGCCGCC
TGGAGGCCCAGAAGAGAGAGCTGCAGCAGGCTGTGCTGCACATGGAGCAG
CGGAAGCAGCAGCAGCAGCAGCAGCAAGGCCACAAGGCCCCGGCTGCCCA
CCCTGAGGGGCAGCTCAAGTTCCACCCAGACACAGACGATGTACCTGTCC
CAGCTCCAGCCGGTGACCAGAAGGAGGTGGACACTTCAGAAAAGAAACTT
CTCGAGCGGCTCCCTGAGGTTGAGGTGCCCCAGCATCTGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAPPSG
PRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYL
QEVIDVLETDGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKR
QEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLE
LLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERK
LEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFIPHDIN
SDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVD
TNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELA
AREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQ
QQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEV
EVPQHL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express NUCB1 with N-terminal GST (contains precission protease site); N.B. Sequence change compared with NM_006184:; A24g (silent in G8), t752c (L251P, a837g (silent in P279); (All changes present in NUCB1 clones made by M. Peggie); Estimated MW of expressed protein : 81902 Daltons