cDNA Clone SLX1 (yeast)

• Plasmid: pFastBacDual SLX1/6His SLX4
• Parent Plasmid: pFastbacDual
• Genbank: Z36097 / Z73307
• Tag: 6His
• Species: Yeast

Sequence of Insert

DNA sequence of insert 1 : ccatggGAAACCATGTCGCAGAAAATACAGCAGCATCAGTTTCCTGATTTTTATTGTTGTTATCTACTACAATCGATTAACAAGAGGCAATCATTTTACGTTGGATCAACTCCTAACCCAGTACGACGTTTGAGGCAGCATAATGGGAAATTGGCTGTTGGAGGGGCATATCGAACCAAACGTGACGGTTCAAGGCCCTGGGAAATGATAATGATTGTGCGGGGCTTCCCAAGTAAGATAGCAGCTTTACAGTTTGAACATGCATGGCAACATGGGTACCAAACGCATTATATTGCCGAAAAAGATCGTGTTGTAAAGCATAAAGCAGGTGGAAGAACTTTACATCACAAAGTTGCATTAATGAAGTTACTTTTAAAGCATGAATTTTTTCAAAGGATGAACTTGATAGTCGAGGTTTTTAATATAAAGGCTTGGGAGGTTTGGAAACAGGACAAATTTTTTATTGAAAGAGACAGATTTCCGATTAATATACAAATAAATGAAAATGCGCTTGAAGAACCAAAGGAGAAAACTGTGGATGTTCTCATGGATCATAGTGATGAGAATTTAAAAGTGGTGGAAGCTGTATACACTAAAGTCATTGAAAATGAA
AGAAACATATTCGAAACTTTCGAAAAGAAACTAACTACAGGGGTAGTGCG
ATGTGAAATTTGTGAGAAAGAAATTGACTACACGTCTGAAGAGCAAAATC
TGAAGCCGTTTGTAGCGCTTTGCAATAATAAAGACTGTGGGTGTGTTAAC
CACTTGAAATGTCTTCACAGGTACTTTCTAGATGATGAACAGTTGATGGT
AGGGAGAAGAAATTTGATACCTCGAGGTGGGAAATGTCCGAAATGTGATA
TGTTTTGCGACTGGACGACCCTAGTCAAGTTTTCGACAAGAATGAAGTTA
GCTCACGGGAAGTAGccatgg

DNA sequence of insert 2 : ggatccGAAACCATGCACCATCACCATCACCATGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTAGGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAGCCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAAAATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAATGCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAAACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATATAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTATTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGACGCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGGGTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATTATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAATGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAGACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCACCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACGCGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATGACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGAGATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACCGGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTATAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTTGAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATATTTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAGACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCCACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTAAACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCCAATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTCTCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAAATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAATATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCTGTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGggatcc

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (p10 promoter) : MSQKIQQHQFPDFYCCYLLQSINKRQSFYVGSTPNPVRRLRQHNGKLAVG
GAYRTKRDGSRPWEMIMIVRGFPSKIAALQFEHAWQHGYQTHYIAEKDRV
VKHKAGGRTLHHKVALMKLLLKHEFFQRMNLIVEVFNIKAWEVWKQDKFF
IERDRFPINIQINENALEEPKEKTVDVLMDHSDENLKVVEAVYTKVIENE
RNIFETFEKKLTTGVVRCEICEKEIDYTSEEQNLKPFVALCNNKDCGCVN
HLKCLHRYFLDDEQLMVGRRNLIPRGGKCPKCDMFCDWTTLVKFSTRMKL
AHGK*

Amino acid sequence of expressed protein 2 (Polyhedrin promoter) : MHHHHHHELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVP
EMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVS
TSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNA
DDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYER
SRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSE
QEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLW
TASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEES
IIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKA
CKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIEND
ERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNK
TGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQ
ESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTK
DSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKP
MRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEA
FPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLR
NDKK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express SLX1 and 6His.SLX4.