cDNA Clone PIM3

• Synonyms: pim-3
• Plasmid: pGEX4T3 PIM3
• Parent Plasmid: pGEX4T3
• Expressed: GST PIM3
• Genbank: Q86V86
• Tag: GST
• Species: Human

Sequence of Insert

gaattcCCTGCTCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCG
GCGGCGTGGACCACCTCCCGGTGAAGATCCTGCAGCCAGCCAAGGCGGAC
AAGGAGAGCTTCGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGGTAGCGG
CGGCTTCGGCACGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGG
TGGCTGTGAAGCACGTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTG
GGCGGCGCGACCGTGCCCCTGGAGGTGGTGCTGCTGCGCAAGGTGGGCGC
GGCGGGCGGCGCGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGC
CCGACGGCTTCCTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTC
TTCGACTTTATCACGGAGCGCGGCGCCCTGGACGAGCCGCTGGCGCGCCG
CTTCTTCGCGCAGGTGCTGGCCGCCGTGCGCCACTGCCACAGCTGCGGGG
TCGTGCACCGCGACATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCC
GGAGAGCTCAAGCTCATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACAC
GGTCTACACCGACTTCGACGGCACCCGAGTGTACAGCCCCCCGGAGTGGA
TCCGCTACCACCGCTACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGC
GTGCTTCTCTACGATATGGTGTGTGGGGACATCCCCTTCGAGCAGGACGA
GGAGATCCTCCGAGGCCGCCTGCTCTTCCGGAGGAGGGTCTCTCCAGAGT
GCCAGCAGCTGATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAGCGGCCG
TCGCTGGATCAGATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGG
CGCCCCGGAGAGCTGTGACCTGCGGCTGTGCACCCTCGACCCTGATGACG
TGGCCAGCACCACGTCCAGCAGCGAGAGCTTGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPNSLLSKFGSLAHLCGPGGVDHLP
VKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVV
KERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLV
LERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIK
DENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYH
GRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRW
CLSLRPSERPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSS
SESL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX4T3 PIM355.72 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU5048
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 62282 Daltons
• Low Temp: No