cDNA Clone PIM1

• Plasmid: pGEX4T-3-PIM1
• Parent Plasmid: pGEX4T3
• Expressed: GST-PIM1
• Genbank: NM_002648
• Tag: GST

Sequence of Insert

gaattcCCTCTTGTCCAAAATCAACTCGCTTGCCCACCTGCGCGCCGCGC
CCTGCAACGACCTGCACGCCACCAAGCTGGCGCCCGGCAAGGAGAAGGAG
CCCCTGGAGTCGCAGTACCAGGTGGGCCCGCTACTGGGCAGCGGCGGCTT
CGGCTCGGTCTACTCAGGCATCCGCGTCTCCGACAACTTGCCGGTGGCCA
TCAAACACGTGGAGAAGGACCGGATTTCCGACTGGGGAGAGCTGCCTAAT
GGCACTCGAGTGCCCATGGAAGTGGTCCTGCTGAAGAAGGTGAGCTCGGG
TTTCTCCGGCGTCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAGTT
TCGTCCTGATCCTGGAGAGGCCCGAGCCGGTGCAAGATCTCTTCGACTTC
ATCACGGAAAGGGGAGCCCTGCAAGAGGAGCTGGCCCGCAGCTTCTTCTG
GCAGGTGCTGGAGGCCGTGCGGCACTGCCACAACTGCGGGGTGCTCCACC
GCGACATCAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTC
AAGCTCATCGACTTCGGGTCGGGGGCGCTGCTCAAGGACACCGTCTACAC
GGACTTCGATGGGACCCGAGTGTATAGCCCTCCAGAGTGGATCCGCTACC
ATCGCTACCATGGCAGGTCGGCGGCAGTCTGGTCCCTGGGGATCCTGCTG
TATGATATGGTGTGTGGAGATATTCCTTTCGAGCATGACGAAGAGATCAT
CAGGGGCCAGGTTTTCTTCAGGCAGAGGGTCTCTTCAGAATGTCAGCATC
TCATTAGATGGTGCTTGGCCCTGAGACCATCAGATAGGCCAACCTTCGAA
GAAATCCAGAACCATCCATGGATGCAAGATGTTCTCCTGCCCCAGGAAAC
TGCTGAGATCCACCTCCACAGCCTGTCGCCGGGGCCCAGCAAATAGgaat
tc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPNSLLSKINSLAHLRAAPCNDLHA
TKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKD
RISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILER
PEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDEN
ILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRS
AAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLA
LRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5038
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 62105 Daltons