cDNA Clone MARK1

• Plasmid: pGEX6P-1-MARK1 2-380 (kinase domain + UBA domain)
• Parent Plasmid: pGEX6P1
• Expressed: GST-MARK1 2-380 (kinase domain + UBA domain)
• Genbank: NM_018650
• Tag: GST
• Species: Human

Sequence of Insert

gaattcTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGA
AAATCATACATCTGTGGATGGATATACTGAACCACACATCCAGCCTACCA
AGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCATTACGTCA
GCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAAT
AGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTG
GTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACC
AGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCA
TCCTAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCT
ATTTAGTCATGGAATACGCGAGTGGGGGTGAAGTATTTGATTACTTAGTT
GCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTAGGCAGAT
TGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATC
TTAAGGCTGAAAACCTTCTCCTTGATGGTGATATGAATATTAAAATTGCT
GACTTTGGTTTTAGTAATGAATTTACAGTTGGGAACAAATTGGACACATT
TTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGT
ATGATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACA
TTAGTCAGTGGCTCCTTGCCTTTCGATGGCCAGAATTTAAAGGAACTGCG
AGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATGTCCACAG
ACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGA
GGCAGCTTGGAACAAATAATGAAAGATCGATGGATGAATGTTGGTCATGA
AGAGGAAGAACTAAAGCCATATACTGAGCCTGATCCGGATTTCAATGACA
CAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATA
AATGATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATAT
TCTTCTAGGTAGAAAACCACCTGAATTTGAAGGTGGTGAATCGTaATCCA
GTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAACAACAGC
ACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAA
TCAGAAGCAGCGGCGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTG
CTGTATCATATACCAAAAGACCTCAGGCTAACAGTGTGGAAAGTGAACAG
AAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGT
TGGATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGA
AAAAATCTTCAACTATTCCAAGTAACAATGTGTATTCTGGAGGTAGCATG
GCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGATACGTAGC
ATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCA
TATCTTCTGCAGGCTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCC
CGCCACCAGAAGTCCATGTCCACTTCTGGTCATCCTATTAAAGTCACACT
GCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGA
GAGTGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCA
GACCGGACCCGTTTTCCCCGAGGGAGCTCAAGCCGAAGCACTTTCCATGG
TGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCACCTGCTT
CACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCA
ACTGGTATAATAAGCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAG
TGAAGGCGAAGCCAGTGGCAGAACCGACACCTCAAGAAGTACATCAGGGG
AACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCT
TTGCGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGA
CATGATGAGAGAAATCCGAAAAGTGTTAGATGCAAATAACTGTGATTATG
AGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGCTAGACAG
GATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTC
ACTTAATGGGGTTCGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTA
AGAACATTGCATCAAAAATAGCAAATGAGCTTAAGCTGTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFSARTPLPTVNERDTEN
HTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIG
KGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHP
NIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIV
SAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFC
GSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRE
RVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEE
EELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYIL
LGRKPPEFEGGES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5036
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Changes to NM_018650 sequence: V16E, T20S – present in EST clones (and MARK1 from other species) and assumed to be ‘correct’ sequence.; Estimated MW of expressed protein : 70543 Daltons