cDNA Clone METTL1

• Plasmid: pEBG6P-1 METTL1 S27A
• Parent Plasmid: pEBG6P
• Expressed: GST-METTL1 S27A
• Genbank: Y18643
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGCAGCCGAGACTCGGAACGTGGCCGGAGCAGAGGCCCCACCGCCCCAGAAGCGCTACTACCGGCAACGTGCTCACGCCAACCCCATGGCGGACCACACGCTGCGCTACCCTGTGAAGCCAGAGGAGATGGACTGGTCTGAGCTATAC
CCAGAGTTCTTCGCTCCACTCACTCAAAATCAGAGCCACGATGACCCAAA
GGATAAGAAAGAAAAGAGAGCTCAGGCCCAAGTGGAGTTTGCAGACATAG
GCTGTGGCTATGGTGGCCTGTTAGTGGAACTGTCACCGCTGTTCCCAGAC
ACACTTATTCTGGGTCTGGAGATCCGGGTGAAGGTCTCAGACTATGTACA
AGACCGGATTCGGGCCCTACGCGCAGCTCCTGCAGGTGGCTTCCAGAACA
TCGCCTGTCTCCGTAGCAATGCCATGAAGCACCTTCCTAACTTCTTCTAC
AAGGGCCAGCTGACAAAGATGTTCTTCCTCTTCCCCGACCCACATTTCAA
GCGGACAAAGCACAAGTGGCGAATCATCAGTCCCACCCTGCTAGCAGAAT
ATGCCTACGTGCTAAGAGTTGGGGGGCTGGTGTATACCATAACCGATGTG
CTGGAGCTACACGACTGGATGTGCACTCATTTCGAAGAGCACCCACTGTT
TGAGCGTGTGCCTCTGGAGGACCTGAGTGAAGACCCCGTTGTGGGACATC
TAGGCACCTCAACTGAGGAGGGGAAGAAAGTTCTACGTAATGGAGGGAAG
AATTTCCCAGCCATCTTCCGAAGAATACAAGATCCCGTCCTCCAGGCAGT
GACCTCCCAAACCAGCCTGCCTGGTCACTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAAETRNVAGAEAP
PPQKRYYRQRAHANPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHD
DPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSD
YVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDP
HFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEH
PLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVL
QAVTSQTSLPGH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express METTL1 S27A with N-terminal GST (contains precission protease site); Estimated MW of expressed proetin : 58720 Daltons