cDNA Clone SLX1 (yeast)

• Plasmid: pETBlue2 6his.SLX1
• Parent Plasmid: pETBlue2 original
• Genbank: Z36097
• Tag: 6His
• Species: Yeast

Sequence of Insert

ccatggGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCGTCGCAGAAAATACAGCAGCATCAGTTTCCTGATTTTTATTGTTGTTATCTACTACAATCGATTAACAAGAGGCAATCATTTTACGTTGGATCAACTCCTAACCCAGTACGACGTTTGAGGCAGCATAATGGGAAATTGGCTGTTGGAGGGGCATATCGAACCAAACGTGACGGTTCAAGGCCCTGGGAAATGATAATGATTGTGCGGGGCTTCCCAAGTAAGATAGCAGCTTTACAGTTTGAACATGCATGGCAACATGGGTACCAAACGCATTATATTGCCGAAAAAGATCGTGTTGTAAAGCATAAAGCAGGTGGAAGAACTTTACATCACAAAGTTGCATTAATGAAGTTACTTTTAAAGCATGAATTTTTTCAAAGGATGAACTTGATAGTCGAGGTTTTTAATATAAAGGCTTGGGAGGTTTGGAAACAGGACAAATTTTTTATTGAAAGAGACAGATTTCCGATTAATATACAAATAAATGAAAATGCGCTTGAAGAACCAAAGGAGAAAACTGTGGATGTTCTCATGGATCATAGTGATGAGAATTTAAAAGTGGTGGAAGCTGTATACACTAAAGTCATTGAAAATGAAAGAAACATATTCGAAACTTTCGAAAAGAAACTAACTACAGGGGTAGTGCGATGTGAAATTTGTGAGAAAGAAATTGACTACACGTCTGAAGAGCAAAATCTGAAGCCGTTTGTAGCGCTTTGCAATAATAAAGACTGTGGGTGTGTTAACCACTTGAAATGTCTTCACAGGTACTTTCTAGATGATGAACAGTTGATGGTAGGGAGAAGAAATTTGATACCTCGAGGTGGGAAATGTCCGAAATGTGATATGTTTTGCGACTGGACGACCCTAGTCAAGTTTTCGACAAGAATGAAGTTAGCTCACGGGAAGTAGGGATCCGAATTCGAGCTCGGCGCGCCTGCAGGTCGACAAGCTTgcggccgc

Amino Acid Sequence

MGSSHHHHHHSQDPSQKIQQHQFPDFYCCYLLQSINKRQSFYVGSTPNPV
RRLRQHNGKLAVGGAYRTKRDGSRPWEMIMIVRGFPSKIAALQFEHAWQH
GYQTHYIAEKDRVVKHKAGGRTLHHKVALMKLLLKHEFFQRMNLIVEVFN
IKAWEVWKQDKFFIERDRFPINIQINENALEEPKEKTVDVLMDHSDENLK
VVEAVYTKVIENERNIFETFEKKLTTGVVRCEICEKEIDYTSEEQNLKPF
VALCNNKDCGCVNHLKCLHRYFLDDEQLMVGRRNLIPRGGKCPKCDMFCD
WTTLVKFSTRMKLAHGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1499
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express SLX1 with N-terminal 6His tag.