cDNA Clone SLX4 (yeast)

• Plasmid: pETBlue2 6his.SLX4
• Parent Plasmid: pETBlue2 original
• Genbank: Z73307
• Tag: 6His
• Species: Yeast

Sequence of Insert

ccatggGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCGGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTAGGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAGCCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAAAATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAATGCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAAACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATATAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTATTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGACGCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGGGTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATTATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAATGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAGACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCACCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACGCGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATGACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGAGATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACCGGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTATAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTTGAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATATTTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAGACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCCACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTAAACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCCAATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTCTCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAAATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCTGTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGGGATCCGAATTCGAGCTCGGCGCGCCTGCAGGTCGACAAGCTTgcggccgc

Amino Acid Sequence

MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSL
GMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVD
KSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKY
SFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDF
NINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKA
TFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQE
SSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSE
IMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAF
SLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYR
YVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISP
LKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSS
IRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVD
SSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSL
KTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDH
LTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWAD
VQGICLRNDKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1498
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express SLX4 with N-terminal 6His tag.; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.