cDNA Clone CDC6

• Plasmid: pGEX6P-1-CDC6
• Parent Plasmid: pGEX6P1
• Expressed: GST-CDC6
• Genbank: NM_001254
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCCTCAAACCCGATCCCAGGCACAGGCTACAATCAGTTTTCCAAAAAGGAAGCTGTCTCGGGCATTGAACAAAGCTAAAAACTCCAGTGATGCCAAACTAGAACCAACAAATGTCCAAACCGTAACCTGTTCTCCTCGTGTAAAAGCC
CTGCCTCTCAGCCCCAGGAAACGTCTGGGCGATGACAACCTATGCAACAC
TCCCCATTTACCTCCTTGTTCTCCACCAAAGCAAGGCAAGAAAGAGAATG
GTCCCCCTCACTCACATACACTTAAGGGACGAAGATTGGTATTTGACAAT
CAGCTGACAATTAAGTCTCCTAGCAAAAGAGAACTAGCCAAAGTTCACCA
AAACAAAATACTTTCTTCAGTTAGAAAAAGTCAAGAGATCACAACAAATT
CTGAGCAGAGATGTCCACTGAAGAAAGAATCTGCATGTGTGAGACTATTC
AAGCAAGAAGGCACTTGCTACCAGCAAGCAAAGCTGGTCCTGAACACAGC
TGTCCCAGATCGGCTGCCTGCCAGGGAAAGGGAGATGGATGTCATCAGGA
ATTTCTTGAGGGAACACATCTGTGGGAAAAAAGCTGGAAGCCTTTACCTT
TCTGGTGCTCCTGGAACTGGAAAAACTGCCTGCTTAAGCCGGATTCTGCA
AGACCTCAAGAAGGAACTGAAAGGCTTTAAAACTATCATGCTGAATTGCA
TGTCCTTGAGGACTGCCCAGGCTGTATTCCCAGCTATTGCTCAGGAGATT
TGTCAGGAAGAGGTATCCAGGCCAGCTGGGAAGGACATGATGAGGAAATT
GGAAAAACATATGACTGCAGAGAAGGGCCCCATGATTGTGTTGGTATTGG
ACGAGATGGATCAACTGGACAGCAAAGGCCAGGATGTATTGTACACGCTA
TTTGAATGGCCATGGCTAAGCAATTCTCACTTGGTGCTGATTGGTATTGC
TAATACCCTGGATCTCACAGATAGAATTCTACCTAGGCTTCAAGCTAGAG
AAAAATGTAAGCCACAGCTGTTGAACTTCCCACCTTATACCAGAAATCAG
ATAGTCACTATTTTGCAAGATCGACTTAATCAGGTATCTAGAGATCAGGT
TCTGGACAATGCTGCAGTTCAATTCTGTGCCCGCAAAGTCTCTGCTGTTT
CAGGAGATGTTCGCAAAGCACTGGATGTTTGCAGGAGAGCTATTGAAATT
GTAGAGTCAGATGTCAAAAGCCAGACTATTCTCAAACCACTGTCTGAATG
TAAATCACCTTCTGAGCCTCTGATTCCCAAGAGGGTTGGTCTTATTCACA
TATCCCAAGTCATCTCAGAAGTTGATGGTAACAGGATGACCTTGAGCCAA
GAAGGAGCACAAGATTCCTTCCCTCTTCAGCAGAAGATCTTGGTTTGCTC
TTTGATGCTCTTGATCAGGCAGTTGAAAATCAAAGAGGTCACTCTGGGGA
AGTTATATGAAGCCTACAGTAAAGTCTGTCGCAAACAGCAGGTGGCGGCT
GTGGACCAGTCAGAGTGTTTGTCACTTTCAGGGCTCTTGGAAGCCAGGGG
CATTTTAGGATTAAAGAGAAACAAGGAAACCCGTTTGACAAAGGTGTTTT
TCAAGATTGAAGAGAAAGAAATAGAACATGCTCTGAAAGATAAAGCTTTA
ATTGGAAATATCTTAGCTACTGGATTGCCTTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPQTRSQAQATISFPKRKLS
RALNKAKNSSDAKLEPTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHL
PPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIKSPSKRELAKVHQNKI
LSSVRKSQEITTNSEQRCPLKKESACVRLFKQEGTCYQQAKLVLNTAVPD
RLPAREREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTACLSRILQDLK
KELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKH
MTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTL
DLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDN
AAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSP
SEPLIPKRVGLIHISQVISEVDGNRMTLSQEGAQDSFPLQQKILVCSLML
LIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILG
LKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1457
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 89356 Daltons