cDNA Clone HUNK

• Plasmid: pFastBac.HTc HUNK
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-HUNK
• Genbank: NM_014586
• Tag: 6His
• Species: Human

Sequence of Insert

actagtCCGGCGGCGGCGGGGGACGGGCTCCTGGGGGAGCCGGCGGCGCCTGGGGGCGGCGGCGGCGCGGAGGACGCGGCCAGGCCCGCGGCGGCCTGCGAGGGAAGTTTCCTGCCTGCCTGGGTGAGCGGCGTGCCCCGCGAGCGGCTCCGCGACTTCCAGCACCACAAGCGCGTGGGCAACTACCTCATCGGCAGCAGGAAGCTGGGCGAGGGCTCCTTTGCCAAGGTGCGCGAGGGGCTGCACGTGCTGACCGGGGAGAAGGTGGCCATAAAAGTCATTGATAAGAAGAGAGCCAAAAAGGACACCTATGTCACCAAAAACCTGCGGCGAGAGGGTCAGATCCAGCAGATGATCCGCCACCCCAATATCACTCAGCTCCTTGATATTTTAGAAACGGAAAACAGCTACTACCTGGTCATGGAGCTGTGCCCTGGGGGCAACCTGATGCACAAGATCTATGAGAAGAAGCGGCTGGAGGAGTCCGAAGCCCGCAGATACATCCGACAGCTCATCTCTGCCGTAGAGCACCTGCACCGGGCCGGGGTGGTCCACAGAGACTTGAAGATAGAGAATTTGCTACTAGATGAAGACAATAATATCAAGCTGATTGACTTTGGTTTGAGCAACTGCGCAGGGATCCTGGGTTACTCGGATCCGTTCAGCACACAGTGTGGCAGCCCTGCCTACGCTGCACCTGAACTGCTCGCCAGGAAGAAATACGGCCCCAAAATCGATGTCTGGTCCATAGGTGTGAACATGTATGCCATGTTGACCGGGACGCTGCCTTTCACGGTGGAGCCTTTCAGCCTGAGGGCTTTGTACCAGAAGATGGTAGACAAAGAAATGAACCCCCTCCCCACTCAGCTCTCCACAGGTGCCATCAGTTTCCTGCGCTCTCTCCTGGAACCGGATCCTGTGAAGAGGCCAAATATTCAGCAGGCACTGGCGAATCGCTGGCTTAATGAGAATTACACGGGCAAAGTGCCCTGTAATGTCACCTATCCCAACAGGATTTCTCTGGAAGATCTGAGCCCGAGCGTCGTGCTGCACATGACCGAGAAGCTGGGTTACAAGAACAGCGACGTGATCAACACTGTGCTCTCCAACCGCGCCTGCCACATCCTGGCCATCTACTTCCTCTTAAACAAGAAACTGGAGCGCTATTTGTCAGGGAAATCTGACATCCAGGACAGCCTCTGCTACAAGACCCGGCTCTACCAGATAGAAAAGTACAGGGCCCCCAAGGAGTCCTATGAGGCCTCTCTGGACACCTGGACACGAGATCTTGAATTCCATGCCGTGCAGGATAAAAAGCCCAAAGAACAAGAAAAAAGAGGGGATTTTCTTCATCGACCATTCTCCAAGAAGTTGGACAAGAACCTGCCCTCGCACAAACAGCCCTCAGGCTCGCTCATGACACAGATTCAGAACACCAAAGCCCTCCTGAAGGACCGGAAGGCCTCCAAGTCCAGCTTCCCCGACAAAGATTCCTTTGGCTGCCGCAATATTTTCCGCAAAACCTCAGATTCCAATTGTGTGGCTTCTTCCTCCATGGAGTTCATCCCCGTGCCACCGCCCAGGACCCCGAGGATTGTGAAGAAACCGGAGCCCCATCAGCCAGGGCCCGGAAGCACTGGCATCCCCCACAAGGAAGACCCCCTGATGCTGGACATGGTGCGCTCCTTCGAGTCTGTGGATCGCGACGACCACGTAGAAGTGCTGTCTCCCTCTCATCACTACAGGATTCTGAACTCCCCGGTCAGCTTGGCTCGCAGAAATTCCAGCGAGAGGACGCTGTCCCCGGGTCTGCCATCCGGAAGCATGTCGCCTCTCCATACTCCTTTGCATCCAACTCTGGTCTCTTTTGCTCACGAAGATAAGAACAGCCCCCCAAAAGAGGAGGGCCTGTGTTGCCCACCTCCGGTTCCCAGCAATGGCCCCATGCAGCCTCTGGGGAGCCCCAATTGTGTGAAAAGCCGAGGCCGGTTCCCTATGATGGGCATCGGACAGATGTTAAGGAAGCGCCATCAGAGTCTGCAGCCATCTGCAGATAGGCCCCTGGAGGCCAGCCTGCCCCCACTGCAGCCCCTAGCCCCTGTGAACCTTGCCTTTGACATGGCCGATGGGGTCAAGACCCAGTGCTAAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDELTSPAAAGDGLLG
EPAAPGGGGGAEDAARPAAACEGSFLPAWVSGVPRERLRDFQHHKRVGNY
LIGSRKLGEGSFAKVREGLHVLTGEKVAIKVIDKKRAKKDTYVTKNLRRE
GQIQQMIRHPNITQLLDILETENSYYLVMELCPGGNLMHKIYEKKRLEES
EARRYIRQLISAVEHLHRAGVVHRDLKIENLLLDEDNNIKLIDFGLSNCA
GILGYSDPFSTQCGSPAYAAPELLARKKYGPKIDVWSIGVNMYAMLTGTL
PFTVEPFSLRALYQKMVDKEMNPLPTQLSTGAISFLRSLLEPDPVKRPNI
QQALANRWLNENYTGKVPCNVTYPNRISLEDLSPSVVLHMTEKLGYKNSD
VINTVLSNRACHILAIYFLLNKKLERYLSGKSDIQDSLCYKTRLYQIEKY
RAPKESYEASLDTWTRDLEFHAVQDKKPKEQEKRGDFLHRPFSKKLDKNL
PSHKQPSGSLMTQIQNTKALLKDRKASKSSFPDKDSFGCRNIFRKTSDSN
CVASSSMEFIPVPPPRTPRIVKKPEPHQPGPGSTGIPHKEDPLMLDMVRS
FESVDRDDHVEVLSPSHHYRILNSPVSLARRNSSERTLSPGLPSGSMSPL
HTPLHPTLVSFAHEDKNSPPKEEGLCCPPPVPSNGPMQPLGSPNCVKSRG
RFPMMGIGQMLRKRHQSLQPSADRPLEASLPPLQPLAPVNLAFDMADGVK
TQC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Estimated MW of expressed protein : 84263 Daltons