cDNA Clone PI3K beta

• Plasmid: pFastBac.HTa PI3K beta
• Parent Plasmid: pSC-A
• Expressed: 6His-PI3K beta
• Genbank: NM_006219
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCT
ACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCC
AATGTTCAACCTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGA
ATCAGACTGCTGTATATGAGGAGCTTGAAGATGAAACACGAAGACTCTGT
GATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGA
CCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGGTC
TGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGA
AAAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTGTGGGATTGTC
TTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCA
TCCCTGAAAACTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCT
GTTCATTTTGAAGACTGCCAGGACGTGTTTAGCTTTCAAGTGTCTCCTAA
TATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTA
TTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTC
AGCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCA
GTATATCCGGAACTGTGTGATGAACAGAGCCCTGCCCCATTTTATACTTG
TGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATA
GAGGCTGCCATAAATCGAAATTCATCTAATCTTCCTCTTCCATTACCACC
AAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACAACCCTTTCC
AAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAA
GTTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAAC
CATCGTAAGCTCAGAGGTATCAGGGAAAAATGATCATATTTGGAATGAAC
CACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTA
TGTTTTGCTGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAAC
GAAAACTATTAATCCCTCTAAATATCAGACCATCAGGAAAGCTGGAAAAG
TGCATTATCCTGTAGCGTGGGTAAATACGATGGTTTTTGACTTTAAAGGA
CAATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGA
TGAACTCGAAGAAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCAT
ATACTGAAAATGCAACAGCTTTGCATGTTAAATTTCCAGAGAATAAAAAA
CAACCTTATTATTACCCTCCCTTCGATAAGATTATTGAAAAGGCAGCTGA
GATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTGGAAAAAAGT
TTCTTCCTGTATTGAAAGAAATCTTGGACAGGGGTCCCTTGTCTCAACTG
TGTGAAAATGAAATGGATCTTATTTGGACTTTGCGACAAGACTGCCGAGA
GATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATA
AACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAA
CTGCCCCCCCGGGAGGCCCTAGAGCTTCTGGATTTCAACTATCCAGACCA
GTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATGAGTGATGAAG
AACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCT
TTTCTTGATTGTGCCCTCTCTAGATTCCTATTAGAAAGGGCACTTGGTAA
TCGGAGGATAGGGCAGTTTCTATTTTGGCATCTTAGGTCAGAAGTGCACA
TTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTTGAAGCATACTGCCGG
GGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAA
TAAGTTAAAAACTTTAAATAGTTTAATCAAACTGAATGCCGTGAAGTTAA
ACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAAACAGAGTGCT
TACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTAT
CCTCTCAGAACTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGA
AGCCTTTGTGGCTGGTATACAATAACAAGGTATTTGGTGAGGATTCAGTT
GGAGTGATTTTTAAAAATGGTGATGATTTACGACAGGATATGTTGACACT
CCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGATC
TTCGGATGTTGCCTTATGGCTGTTTAGCAACAGGAGATCGCTCTGGCCTC
ATTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTCAGCTGAACAG
TAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACT
GGCTTAAAGAATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAA
TTTACACTGTCCTGTGCTGGCTACTGTGTAGCTTCTTATGTCCTTGGGAT
TGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTGGCCAGCTCT
TCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGC
ATTAAAAGGGAGCGAGTGCCTTTTATTCTTACCTATGATTTCATCCATGT
CATTCAACAAGGAAAAACAGGAAATACAGAAAAGTTTGGCCGGTTCCGCC
AGTGTTGTGAGGATGCATATCTGATTTTACGACGGCATGGGAATCTCTTC
ATCACTCTCTTTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATC
AGTCAAAGATATACAGTATCTTAAGGACTCTCTTGCATTAGGGAAGAGTG
AAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGG
GAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGA
CTACAGATCTTAAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFCFSFIMPPAMADILDIWAVD
SQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFNL
LMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEK
LDSKIGVLIGKGLHEFDSLKDPEVNEFRRKMRKFSEEKILSLVGLSWMDW
LKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFEDCQDVFSFQVSPNMNPI
KVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRN
CVMNRALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTR
IISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVSS
EVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTIN
PSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELEE
MLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPPFDKIIEKAAEIASS
DSANVSSRGGKKFLPVLKEILDRGPLSQLCENEMDLIWTLRQDCREIFPQ
SLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVRE
YAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIG
QFLFWHLRSEVHIPAVSVQFGVILEAYCRGSVGHMKVLSKQVEALNKLKT
LNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSEL
YVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLR
LMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVA
AAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRH
SDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQG
KTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDI
QYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1434
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence differences compared with NM_006219 CDS:; a613g (N205D); a1634g (D545G); a1989g (silent in R663); Estimated MW of expressed protein : 126208