cDNA Clone PKC zeta

• Plasmid: pCMV5 PKC zeta.6his
• Parent Plasmid: pCMV5
• Expressed: PKC zeta-6His
• Genbank: NM_002744
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcGCCACCATGCCCAGCAGGACCGGCCCCAAGATGGAAGGGAGCGG
CGGCCGCGTCCGCCTCAAGGCGCATTACGGGGGGGACATCTTCATCACCA
GCGTGGACGCCGCCACGACCTTCGAGGAGCTCTGTGAGGAAGTGAGAGAC
ATGTGTCGTCTGCACCAGCAGCACCCGCTCACCCTCAAGTGGGTGGACAG
CGAAGGTGACCCTTGCACGGTGTCCTCCCAGATGGAGCTGGAAGAGGCTT
TCCGCCTGGCCCGTCAGTGCAGGGATGAAGGCCTCATCATTCATGTTTTC
CCGAGCACCCCTGAGCAGCCTGGCCTGCCATGTCCGGGAGAAGACAAATC
TATCTACCGCCGGGGAGCCAGAAGATGGAGGAAGCTGTACCGTGCCAACG
GCCACCTCTTCCAAGCCAAGCGCTTTAACAGGAGAGCGTACTGCGGTCAG
TGCAGCGAGAGGATATGGGGCCTCGCGAGGCAAGGCTACAGGTGCATCAA
CTGCAAACTGCTGGTCCATAAGCGCTGCCACGGCCTCGTCCCGCTGACCT
GCAGGAAGCATATGGATTCTGTCATGCCTTCCCAAGAGCCTCCAGTAGAC
GACAAGAACGAGGACGCCGACCTTCCTTCCGAGGAGACAGATGGAATTGC
TTACATTTCCTCATCCCGGAAGCATGACAGCATTAAAGACGACTCGGAGG
ACCTTAAGCCAGTTATCGATGGGATGGATGGAATCAAAATCTCTCAGGGG
CTTGGGCTGCAGGACTTTGACCTAATCAGAGTCATCGGGCGCGGGAGCTA
CGCCAAGGTTCTCCTGGTGCGGTTGAAGAAGAATGACCAAATTTACGCCA
TGAAAGTGGTGAAGAAAGAGCTGGTGCATGATGACGAGGATATTGACTGG
GTACAGACAGAGAAGCACGTGTTTGAGCAGGCATCCAGCAACCCCTTCCT
GGTCGGATTACACTCCTGCTTCCAGACGACAAGTCGGTTGTTCCTGGTCA
TTGAGTACGTCAACGGCGGGGACCTGATGTTCCACATGCAGAGGCAGAGG
AAGCTCCCTGAGGAGCACGCCAGGTTCTACGCGGCCGAGATCTGCATCGC
CCTCAACTTCCTGCACGAGAGGGGGATCATCTACAGGGACCTGAAGCTGG
ACAACGTCCTCCTGGATGCGGACGGGCACATCAAGCTCACAGACTACGGC
ATGTGCAAGGAAGGCCTGGGCCCTGGTGACACAACGAGCACTTTCTGCGG
AACCCCGAATTACATCGCCCCCGAAATCCTGCGGGGAGAGGAGTACGGGT
TCAGCGTGGACTGGTGGGCGCTGGGAGTCCTCATGTTTGAGATGATGGCC
GGGCGCTCCCCGTTCGACATCATCACCGACAACCCGGACATGAACACAGA
GGACTACCTTTTCCAAGTGATCCTGGAGAAGCCCATCCGGATCCCCCGGT
TCCTGTCCGTCAAAGCCTCCCATGTTTTAAAAGGATTTTTAAATAAGGAC
CCCAAAGAGAGGCTCGGCTGCCGGCCACAGACTGGATTTTCTGACATCAA
GTCCCACGCGTTCTTCCGCAGCATAGACTGGGACTTGCTGGAGAAGAAGC
AGGCGCTCCCTCCATTCCAGCCACAGATCACAGACGACTACGGTCTGGAC
AACTTTGACACACAGTTCACCAGCGAGCCCGTGCAGCTGACCCCAGACGA
TGAGGATGCCATAAAGAGGATCGACCAGTCAGAGTTCGAAGGCTTTGAGT
ATATCAACCCATTATTGCTGTCCACCGAGGAGTCGGTGCACCATCACCATCACCATTGAggtacc

Amino Acid Sequence

MPSRTGPKMEGSGGRVRLKAHYGGDIFITSVDAATTFEELCEEVRDMCRL
HQQHPLTLKWVDSEGDPCTVSSQMELEEAFRLARQCRDEGLIIHVFPSTP
EQPGLPCPGEDKSIYRRGARRWRKLYRANGHLFQAKRFNRRAYCGQCSER
IWGLARQGYRCINCKLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNE
DADLPSEETDGIAYISSSRKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQ
DFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTE
KHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPE
EHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKE
GLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSP
FDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKER
LGCRPQTGFSDIKSHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDT
QFTSEPVQLTPDDEDAIKRIDQSEFEGFEYINPLLLSTEESVHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1373
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_002744 CDS: a17g (results in residue difference D6G).; This change is present in all ESTs and synthetic PRKCZ construct AAP36798, therefore assumed to be the ‘correct’ sequence.