cDNA Clone ILKAP

• Plasmid: pGEX6P-1-ILKAP
• Parent Plasmid: pGEX6P1
• Expressed: GST-ILKAP
• Genbank: NM_030768
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGACCTCTTCGGGGACCTGCCGGAGCCCGAGCGCTCGCCGCGCCC
GGCTGCCGGGAAAGAAGCTCAGAAAGGACCCCTGCTCTTTGATGACCTCC
CTCCGGCCAGCAGTACTGACTCAGGATCAGGGGGACCTTTGCTTTTTGAT
GATCTCCCACCCGCTAGCAGTGGCGATTCAGGTTCTCTTGCCACATCAAT
ATCCCAGATGGTAAAGACTGAAGGGAAAGGAGCAAAGAGAAAAACCTCCG
AGGAAGAGAAGAATGGCAGTGAAGAGCTTGTGGAAAAGAAAGTTTGTAAA
GCCTCTTCGGTGATCTTTGGTCTGAAGGGCTATGTGGCTGAGCGGAAGGG
TGAGAGGGAGGAGATGCAGGATGCCCACGTCATCCTGAACGACATCACCG
AGGAGTGTAGGCCCCCATCGTCCCTCATTACTCGGGTTTCATATTTTGCT
GTTTTTGATGGACATGGAGGAATTCGAGCCTCAAAATTTGCTGCACAGAA
TTTGCATCAAAACTTAATCAGAAAATTTCCTAAAGGAGATGTAATCAGTG
TAGAGAAAACCGTGAAGAGATGCCTTTTGGACACTTTCAAGCATACTGAT
GAAGAGTTCCTTAAACAAGCTTCCAGCCAGAAGCCTGCCTGGAAAGATGG
GTCCACTGCCACGTGTGTTCTGGCTGTAGACAACATTCTTTATATTGCCA
ACCTCGGAGATAGTCGGGCAATCTTGTGTCGTTATAATGAGGAGAGTCAA
AAACATGCAGCCTTAAGCCTCAGCAAAGAGCATAATCCAACTCAGTATGA
AGAGCGGATGAGGATACAGAAGGCTGGAGGAAACGTCAGGGATGGGCGTG
TTTTGGGCGTGCTAGAGGTGTCACGCTCCATTGGGGACGGGCAGTACAAG
CGCTGCGGTGTCACCTCTGTGCCCGACATCAGACGCTGCCAGCTGACCCC
CAATGACAGGTTCATTTTGTTGGCCTGTGATGGGCTCTTCAAGGTCTTTA
CCCCAGAAGAAGCCGTGAACTTCATCTTGTCCTGTCTCGAGGATGAAAAG
ATCCAGACCCGGGAAGGGAAGTCCGCAGCCGACGCCCGCTACGAAGCAGC
CTGCAACAGGCTGGCCAACAAGGCGGTGCAGCGGGGCTCGGCCGACAACG
TCACTGTGATGGTGGTGCGGATAGGGCACTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1365
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion