cDNA Clone ESC4 (yeast)

• Plasmid: pFastBac.HTb yESC4
• Parent Plasmid: pFastBac HTb
• Expressed: 6His yeast ESC4
• Genbank: U10397
• Tag: 6His
• Species: Yeast

Sequence of Insert

ggatccAGCACATCACTTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAATAACTGTCAAATATACGAGCTTTATAACGAAAATTTG
AAAGATGTCAAGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCA
AACAGTACATTTTGTCATTAGTAATACAATCAACTTTCCTTTTTATAAGA
TAGTTTATTTCGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAG
GATTCGGTCAAGACGAAGAGACATTTGAGGACAAATATGTATTCACCTAA
CCCATTTCATTTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGT
TTAATAAGTGTGAATACATATTATACTCTGATTTGCTTCATTTATTAGGA
GGCACTTTAGTCAATTACATCTCAAATAGAACGACGCATGTGATTGTTCA
AAGTCCCCAGGACCCGATCATAGCAACGGTCAGTAAATTGACATTTGGCT
CTTTTTCCTCTTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAG
TGGAAATTTGTCTATCCAATATGGATATTATATCATTTCAAAATGGCGAA
GCCCTTGAAGGGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACA
CCAGTGAGGAACAGTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAAG
CAGACATCATCATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAA
AAATCACCACTTTGCTATCTCACCAGATTTAAACTTTTTCACACCTTTGT
ATTGGTTTTTGAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCG
TTGAGTTTTTCTGACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATAT
TGACTGTTATATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACCA
AAAGCATTAAACCGGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTAT
ATGTTTGCGCTGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTCA
CCAACCATTTCATGCGAAATTATTTACTTCTAAGGAATTAACAGTCGCGT
ACACTAATTATTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAA
ATATTAGGAGGACTTAGCACACCTGAACTAACAAGAAAAAACACACACTT
GATCACGAAGAGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGT
CACTGGACCCGCAAAATGCCATTATTGTGACAAATCATATGTGGTTAGAG
CAATGTTATATGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCA
AAATTTTAAATTGGATGATAACATGGGATGGAATATCGGCCAAATAGGAA
TGGATCATTCATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTAC
GACACGCAATCCATATCAGAGAAGCCGCCTCCCACAAATGATCAAATGGA
TCAAATTAACGATAACACCAATGTATTATCAAAGAAAGATGGTACGCCCA
TTAGTAGTTTTGAGAACTCAATTGATGAAAAGATTGACAAATTACAAAAA
ATATCAGGAGAGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTT
TGTTTCCAGACCTTCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAAT
CTAATTTACAAAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGAG
GTTTTCTGTGAAGGACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAACC
TATTACGGAGTATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCAC
GCAAGAAGAATGACATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCTG
CAAGTTCAGCTAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGAA
AAATGAAAAGGAGAAAGAAACCAATGAATGTCACATTGAAGTAGATCAAA
TGACGAATGAAAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACG
GAAGACGTCACTTCTAAAAAAGATACAGATAAATTCTCGCATTTGTTTGA
AGGATTATCTGATAATGATGATCACATTAATGACGAAAAACCGGCAGTTA
ATTCCAAATATACCACACCCAAGACGTCTCAAAATATTACGAGTGGCGTT
GATACTCCAACCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAA
TTCAAGGTTAGCAAAGACACAAGCTGCGAAGAGATTGCATACAGATATTG
AATCATTAAATGAATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGC
GAGGAGATTTCATTGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAGA
ACTGGCTACTAAAGCGGAAAAGATATTAGCTAGATTTAATGAACTACCAA
ATTATGACCTAAAAGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAAT
GAGGTTGACATCGAAATATTGAACCAACTAGGTATAAAAATTTTTGACAA
TATTAAAGAAACGGATAAATTGAATTGTATTTTTGCTCCAAAGATTTTGA
GAACCGAGAAATTTTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCA
TTGAAACCTGAATTTATTATTGATTTACTGAAACAAATTCATTCTAAAAA
AGATAAACTTTCTCAAATAAATATAAACTTGTTTGATTATGAGATAAATG
GAATTAATGAGAGTATAATATCAAAAACCAAACTACCTACTAAGGTGTTC
GAAAGAGCGAATATTAGATGCATTAACTTAGTCAATGATATACCGGGTGG
TGTAGATACAATAGGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATCA
ACGTTTTACGTAGTAAGAAATGCACATTTGAAGACATAATCCCAAATGAC
GTTTCTAAGCAGGAAAACGGAGGCATTTTTAAATACGTACTTATCGTCAC
CAAAGCTTCACAGGTTAAAAAATTTACCAAACTGATTAATGATCGTGATA
AAAACGAAACAATTTTGATTGTTGAATGGAATTGGTGTGTTGAATCAATT
TTTCATCTCAATGTTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAA
GAAAAATAACTAAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1320
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee