cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pEBG-2T-ERK8 Y177F 6his
• Parent Plasmid: pEBG2T
• Expressed: GST-ERK8 Y177F 6His
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTGCACCGTAGTTTACCCTCGCATTGTCCGGAGATACCTACTCAG
GCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACC
GGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGG
GATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGA
GTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAG
AGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTG
AACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTC
CATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACG
TTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGC
ACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCC
TGAGGGGCCTGAGGACCAGGCCGTGACAGAGTTCGTGGCCACACGCTGGT
ACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTG
GACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACC
CCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGA
CCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGC
CGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGA
TGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGC
GACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTG
CAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACG
AGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGC
CTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGC
AGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGC
ACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTG
TGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCC
GAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGC
TCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAGGGCCCCCTGGGG
CGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAGGCGGGAGG
GGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCA
GGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCA
GCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGG
AGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGC
TTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCAC
TGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGCACCATCACCATCAC
CATTGAGAATTCCCGGGTCGACTCGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEFVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGEGPPGAKEAPPLTLSLVKPGGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEGHHVHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1287
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express ERK8 (Y177F) with N terminal GST and C terminal 6his.; ; Changes to AY065978 CDS sequence:; g15t, silent in V5; a1285g, R429G; a1339g, S447G