cDNA Clone BRSK2

• Plasmid: pFastBac.HTb BRSK2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-BRSK2
• Genbank: AF533878
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGGCTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGTGGCCATCAAGATCGTC
AACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCGGGAGAT
CGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGACG
TTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGT
GGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGA
GGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACA
GCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTGCTGGAC
GAGAAGAACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGT
TGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCACTACGCCTGCC
CCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGG
AGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGA
CGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCC
ACATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCTACGGGGCATG
ATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACA
CATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGCCGATTC
CTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCC
GACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAA
GCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTT
ACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAGGATGAG
GACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGTGGACTC
CCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAATCCATGG
AGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCC
ATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTC
CTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACC
CCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAAGGGGACACCTGTCCAC
ACGCCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAG
CCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAACTCCATCAAGA
ACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCG
ACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGC
GAAGAAGTCCTGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGA
TCTTCGTGGTCATCAAAGACAAACCTCTGAGCTCCATCAAGGCTGACATC
GTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCCCA
AACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCC
AGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAG
GCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGG
CCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGC
TGAGCACACACGACCCGCCTGCGGCCCAGCACTTGTCAGAACCCCCCCCA
CCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGC
CACCAGCTACGAGAGTAGCCTCTGAggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSTSTGKDGGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1280
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; Changes to AF533878 CDS sequence:; g752t (S251I)*, c846g (silent), a1485g (silent)*; *present in BRSK2 sequence in Sugen database