cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pGEX6P-1-ERK8 6his Y177F
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK8-6His Y177F
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTGCACCGTAGTTGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGGGAT
AAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTT
TGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGA
ACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAAC
GCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCAT
CTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTG
TGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGCACA
GTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCCTGA
GGGGCCTGAGGACCAGGCCGTGACAGAGTTCGTGGCCACACGCTGGTACC
GAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGAC
ATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCT
GTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCA
TCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGT
GCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGATGC
CCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGCGAC
TCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAG
CACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGA
GGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGCCTG
AGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGC
GGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACA
CCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTGTGC
AGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAG
CACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCC
CCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAGGGCCCCCTGGGGCGA
AGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAGGCGGGAGGGGA
GCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGC
CCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCG
TACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGG
ATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTT
GCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGG
GCCACCTGCCCCTGCTGGAGGGGCACCATGTGCACCATCACCATCACCATTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEFVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGEGPPGAKEAPPLTLSLVKPGGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEGHHVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1279
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express ERK8 Y177F with N- terminal GST and C-terminal 6his; Changes to AY065978 CDS sequence:; g15t, silent in V5; a1285g, R429G; a1339g, S447G