cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pGEX6P-1-ERK8 6his T175A
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK8-6HisT175A
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTGCACCGTAGTTGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGGGAT
AAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTT
TGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAGAGA
ACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAAC
GCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCAT
CTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTG
TGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGCACA
GTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCCTGA
GGGGCCTGAGGACCAGGCCGTGGCAGAGTACGTGGCCACACGCTGGTACC
GAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGAC
ATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCT
GTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCA
TCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGT
GCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGATGC
CCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGCGAC
TCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAG
CACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGA
GGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGCCTG
AGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGC
GGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACA
CCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTGTGC
AGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAG
CACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCC
CCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAGGGCCCCCTGGGGCGA
AGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAGGCGGGAGGGGA
GCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGC
CCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCG
TACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGG
ATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTT
GCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGG
GCCACCTGCCCCTGCTGGAGGGGCACCATGTGCACCATCACCATCACCATTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVAEYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGEGPPGAKEAPPLTLSLVKPGGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEGHHVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1278
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee