cDNA Clone NUAK2

• Plasmid: pFastBac.HTa NUAK2
• Parent Plasmid: pSC-A
• Expressed: 6His-NUAK2
• Genbank: NM_030952
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCACAACCTGCGG
CACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGT
GAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCC
GGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAG
ATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGA
AGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCC
GGGGCGACCTTTATGACTACATCAGCGAGCGGCAGCAGCTCAGTGAGCGC
GAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGCACTATTGCCA
TCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGG
ATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTAC
CATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTC
GCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCT
GGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTT
GATGGGCATGACCATAAGATCCTAGTGAAACAGATCAGCAACGGGGCCTA
CCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATCCGGTGGCTGT
TGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCAC
TGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCC
GCATGAGGGTGGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTG
ACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTG
TGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGG
CCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAGAATGACATGG
CCCAGTCCCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGC
AAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGC
CTCTGCAGAAGGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTG
CGAGCCCAGGGCAGGCTGCCCCCCTGCTCCCCAAGAAGGGCATTCTCAAG
AAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGA
ATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCA
AGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTGCTCCTCCATCGCAAAGGC
ATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGGAGCTCGCGGC
CCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGG
CCCGGGCCAGCCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCC
TCTGAGTCCTTTGACCAGCTGGACTTGCCTGAACGGCTCCCAGAGCCCCC
ACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCC
CCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTG
GGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTA
CCGACAGGCACTGAGGGTCTGCTCAAAGCTCACCTGAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1275
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; Changes to NM_030952 CDS sequence: t1158c, g1323c – both silent