cDNA Clone MARK1

• Plasmid: pFastBac.HTa MARK1
• Parent Plasmid: pSC-A
• Expressed: 6His-MARK1
• Genbank: NM_018650
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGA
AAATCATACATCTGTGGATGGATATACTGAACCACACATCCAGCCTACCA
AGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCATTACGTCA
GCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAAT
AGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTG
GTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACC
AGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCA
TCCTAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCT
ATTTAGTCATGGAATACGCGAGTGGGGGTGAAGTATTTGATTACTTAGTT
GCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTAGGCAGAT
TGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATC
TTAAGGCTGAAAACCTTCTCCTTGATGGTGATATGAATATTAAAATTGCT
GACTTTGGTTTTAGTAATGAATTTACAGTTGGGAACAAATTGGACACATT
TTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGT
ATGATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACA
TTAGTCAGTGGCTCCTTGCCTTTCGATGGCCAGAATTTAAAGGAACTGCG
AGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATGTCCACAG
ACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGA
GGCAGCTTGGAACAAATAATGAAAGATCGATGGATGAATGTTGGTCATGA
AGAGGAAGAACTAAAGCCATATACTGAGCCTGATCCGGATTTCAATGACA
CAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATA
AATGATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATAT
TCTTCTAGGTAGAAAACCACCTGAATTTGAAGGTGGTGAATCGTTATCCA
GTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAACAACAGC
ACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAA
TCAGAAGCAGCGGCGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTG
CTGTATCATATACCAAAAGACCTCAGGCTAACAGTGTGGAAAGTGAACAG
AAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGT
TGGATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGA
AAAAATCTTCAACTATTCCAAGTAACAATGTGTATTCTGGAGGTAGCATG
GCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGATACGTAGC
ATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCA
TATCTTCTGCAGGCTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCC
CGCCACCAGAAGTCCATGTCCACTTCTGGTCATCCTATTAAAGTCACACT
GCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGA
GAGTGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCA
GACCGGACCCGTTTTCCCCGAGGGAGCTCAAGCCGAAGCACTTTCCATGG
TGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCACCTGCTT
CACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCA
ACTGGTATAATAAGCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAG
TGAAGGCGAAGCCAGTGGCAGAACCGACACCTCAAGAAGTACATCAGGGG
AACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCT
TTGCGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGA
CATGATGAGAGAAATCCGAAAAGTGTTAGATGCAAATAACTGTGATTATG
AGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGCTAGACAG
GATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTC
ACTTAATGGGGTTCGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTA
AGAACATTGCATCAAAAATAGCAAATGAGCTTAAGCTGTAAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPSNNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGPPASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEEGKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Changes to NM_018650 sequence: V16E, T20S – present in EST clones (and MARK1 from other species) and assumed to be ‘correct’ sequence.