cDNA Clone MARK1

• Synonyms: MARK; Par-1c; Par1c
• Plasmid: pCMV5-HA MARK1
• Parent Plasmid: pCMV5
• Expressed: HA-MARK1
• Genbank: NM_018650.4 GI:554790304
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGATATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCATTACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCCTAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGTGGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTAGGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAACCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGGAACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATGATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTTCGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATGTCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAACAAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGATCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAATGATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTGAATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAACAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGGCGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACAGTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGGATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGTAACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGATACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGGCTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCATCCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAGTGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGGGAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCACCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAAGCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTCAAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTGCGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAGTGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGCTAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTTCGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTAAGCTGTAAGTCGAC

Amino Acid Sequence

MYPYDVPDYASARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIP
RCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKII
DKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASG
GEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLD
GDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVW
SLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKL
LVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVT
MGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSR
PSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTKRPQ
ANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPSN
NVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVA
SAVPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGTTQRVPAASPSA
HSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGPPASPSHETGAF
AHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEE
GKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLF
CVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIAN
ELKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5-HA MARK157.5 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No