cDNA Clone UBXD7

• Plasmid: pGEX-TEV-FLAG UBXD7 del. UBA Domain No ATG
• Parent Plasmid: pGEX TEV TEV
• Expressed: GST-FLAG UBXD7 Del. UBA Domain
• Genbank: NM_015562
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATCGCTGAAGAGCCCAGTACCAGTTCAGCAAGTGTCTCTACTGT
CAGACCACACACAGAAGAAGAAGTTCGTGCCCCAATTCCTCAAAAGCAGG
AAATACTGGTGGAACCAGAACCATTATTTGGTGCTCCTAAAAGACGACGG
CCTGCACGTTCAATTTTTGATGGTTTCCGGGATTTTCAGACTGAAACTAT
TCGGCAAGAACAAGAATTAAGAAATGGAGGAGCTATCGATAAGAAATTAA
CTACCCTTGCAGATCTATTCCGGCCACCCATTGATTTGATGCATAAAGGC
AGCTTTGAAACAGCCAAAGAGTGTGGCCAGATGCAAAATAAGTGGCTGAT
GATAAACATTCAAAATGTTCAAGACTTTGCATGTCAGTGCCTCAACCGCG
ATGTGTGGAGCAACGAAGCTGTGAAGAATATTATCCGGGAACATTTCATT
TTCTGGCAGGTTTATCATGACAGTGAGGAAGGTCAGAGATACATACAGTT
TTATAAGTTAGGGGATTTCCCCTATGTTTCCATATTGGACCCACGGACAG
GTCAGAAGCTAGTAGAATGGCACCAGTTAGATGTATCTTCTTTCTTGGAC
CAAGTGACGGGATTTCTGGGTGAACATGGACAACTGGATGGACTTTCTAG
CAGTCCCCCCAAAAAATGTGCCCGTTCCGAGAGCCTTATAGATGCAAGTG
AAGACAGCCAGCTAGAAGCTGCCATCAGAGCCTCCTTACAAGAAACACAT
TTTGATTCAACACAGACAAAACAGGATAGCCGCTCAGATGAAGAATCTGA
ATCTGAACTTTTTTCTGGCAGTGAGGAGTTCATATCCGTTTGTGGCTCTG
ATGAAGAAGAAGAGGTAGAGAATCTTGCCAAGTCCAGAAAGTCTCCCCAC
AAAGATTTGGGGCATAGAAAAGAGGAGAATAGAAGGCCGCTGACTGAGCC
ACCAGTCAGAACTGATCCTGGAACAGCCACAAACCACCAAGGATTGCCAG
CTGTGGATTCAGAGATACTGGAGATGCCACCTGAAAAAGCAGATGGAGTA
GTGGAGGGGATAGATGTAAATGGACCAAAAGCACAGCTGATGTTGCGGTA
TCCAGATGGAAAAAGGGAACAGATCACTCTTCCAGAGCAAGCTAAACTGC
TAGCTTTGGTGAAGCACGTGCAGTCTAAAGGATACCCAAATGAACGTTTT
GAACTTCTCACCAACTTTCCTCGAAGGAAATTATCTCATCTGGACTATGA
TATTACATTGCAAGAGGCAGGCCTTTGTCCTCAAGAGACTGTCTTTGTAC
AGGAAAGAAATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQDHPPKSDENLYFQGGSDYKDDDDKGSIAEEPSTSSASVSTVRPHTEEEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTTLADLFRPPIDLMHKGSFETAKECGQMQNKWLMINIQNVQDFACQCLNRDVWSNEAVKNIIREHFIFWQVYHDSEEGQRYIQFYKLGDFPYVSILDPRTGQKLVEWHQLDVSSFLDQVTGFLGEHGQLDGLSSSPPKKCARSESLIDASEDSQLEAAIRASLQETHFDSTQTKQDSRSDEESESELFSGSEEFISVCGSDEEEEVENLAKSRKSPHKDLGHRKEENRRPLTEPPVRTDPGTATNHQGLPAVDSEILEMPPEKADGVVEGIDVNGPKAQLMLRYPDGKREQITLPEQAKLLALVKHVQSKGYPNERFELLTNFPRRKLSHLDYDITLQEAGLCPQETVFVQERN

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU21294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Differs from reference sequence in a single silent mutation only – a834c; ; Estimated MW of expressed protein : 78818 Daltons; Parental: pGEX TEV FLAG (SC21250)