cDNA Clone IKK beta

• Plasmid: pCMV5-FLAG-3 IKK beta K44A
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG IKK beta K44A
• Genbank: AF080158 (gi 14738764)
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGCCTGG
GAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCCGATG
GCACAATCAGGAAACAGGTGAGCAGATTGCCATCGCGCAGTGCCGGCAGG
AGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGATCATG
AGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTGAGGG
GATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAGTACT
GCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTGCTGT
GGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCTCTGC
GCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAGCCAG
AAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAATTATT
GACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACATCATT
CGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAGAAGT
ACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGAGTGC
ATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGTGGCA
TTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAAGACT
TGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAATCTT
AACAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTGATGCTGAT
GTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAATGGCT
GCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATATCTTG
AACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATGAGAG
TCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCAGAGG
AGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGATAAG
CCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACACATT
GGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTATGAGA
CTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCTTCAA
GAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGGGCCA
GGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTGCAGC
AGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTGCCTC
TCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGGCCAA
GTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTACAGCG
AGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTGGAGG
GAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGAAACT
CCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTACAGA
GGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGAGGAG
CAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACCAGCG
AACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCAATTC
AGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAAAACT
GTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAGAGGT
GGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAGGAGA
AGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAAGGTC
CGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGACTTAG
CCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGCTTAC
CTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAACCTC
TGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAGACCA
GAGTTTCACGCCCTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDSSGRSWSPSLTTQTCGAWEMKERLGTGGFGN
VIRWHNQETGEQIAIAQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARD
VPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSD
IASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSL
CTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQP
VQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWLQ
LMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVT
EDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNE
GHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRK
VWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQ
LKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGREN
EVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKP
RDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPK
VEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNA
SRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVR
EQDQSFTP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20279
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG for mammalian expression. Protein is truncated at residue 742 in comparison to genbank and has an extra P at C terminus. Also differs at A260S. (gi 14738764 revised) ; ; Estimated MW of expressed protein : 86612 Daltons