cDNA Clone SAE2

• Plasmid: pETDuet-1 6-His Precission SAE2
• Parent Plasmid: pETDuet1
• Expressed: 6His Precission SAE2
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGCACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCAAT
GGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGGGG
GCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCAAG
AATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGATAC
TATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACATG
TTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTTAC
CCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGACTA
TAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGATA
ACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATGTT
CCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACTAT
CAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCAGA
GAACCTTTCCTGGCTGTACAATTCGTAACACACCTTCAGAACCTATACAT
TGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAAGA
TGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTGGG
AACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTGAC
ATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGATCC
AGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTTGA
CAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACTGG
GCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAGAA
TGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAGCT
ATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAGCA
GAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCTGC
AATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAGTA
TGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTATT
CCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTGGA
AGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTTTT
TGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCACTG
GATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTGAC
TGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAAGA
TAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAGAT
GGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCTAA
TAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCTTC
AAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTCAT
AGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCCCC
GGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAACCA
ATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGCAA
GATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAATGC
CGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAAAG
AGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTGAT
GATGTCATAGCATTAGATTGA

Amino Acid Sequence

PWHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 73198 Daltons