cDNA Clone RanBP5

• Synonyms: IPO5
• Plasmid: pEBG6P-RanBP5
• Parent Plasmid: pEBG6P
• Expressed: GST-RanBP5
• Genbank: NM_002271.4 GI:51873051
• Tag: GST
• Species: Human

Sequence of Insert

ggatctcgagctcaagcttcgaattctgcagtcgacATGCCTGAGGATCA
AGTTGGAAAACTAGAAGCAACAGAAAACACAATAAGCGCAATGGCGGCGG
CCGCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGC
CCCGACAATGTGGTCCGGAAACAGGCAGAGGAAACCTATGAGAATATCCC
AGGCCAGTCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACAACAG
CTGCTGAAGAGGCTAGACAAATGGCCGCCGTTCTCCTAAGACGTCTCTTG
TCCTCTGCATTTGATGAAGTCTATCCAGCACTTCCCTCTGATGTTCAGAC
TGCCATCAAGAGTGAGCTACTCATGATTATTCAGATGGAAACACAATCTA
GCATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTA
ATAGATGAGGATGGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTT
TGATTCAGTCAGCTCTCAAAATGTGGGACTGCGGGAAGCTGCCCTTCACA
TTTTCTGGAACTTTCCTGGAATTTTTGGGAACCAGCAACAACACTATTTA
GATGTCATCAAACGAATGTTAGTTCAGTGTATGCAAGATCAGGAACACCC
GTCGATCAGGACGTTATCTGCTAGAGCTACAGCTGCATTTATACTTGCAA
ATGAGCATAATGTTGCTCTGTTCAAACATTTTGCAGACTTGCTACCGGGA
TTCCTACAGGCGGTAAATGACTCGTGCTACCAGAATGATGATTCTGTCCT
AAAATCCCTCGTTGAGATTGCAGATACTGTTCCAAAGTATTTGCGTCCTC
ACTTGGAAGCAACTCTACAGCTAAGTCTAAAGTTGTGTGGAGACACTAGC
CTCAACAATATGCAACGCCAGCTTGCCCTTGAAGTGATCGTCACCCTCTC
TGAGACTGCAGCTGCTATGTTAAGAAAACATACCAATATTGTTGCACAGA
CTATTCCTCAGATGTTAGCAATGATGGTTGATTTGGAAGAAGATGAGGAC
TGGGCAAATGCAGATGAACTAGAAGATGATGATTTTGACAGCAATGCAGT
TGCAGGCGAGAGTGCTCTAGATCGAATGGCTTGCGGACTTGGTGGAAAGC
TCGTTCTGCCGATGATCAAGGAACACATTATGCAAATGCTTCAAAATCCT
GACTGGAAATACCGGCATGCAGGATTGATGGCCTTATCTGCCATTGGTGA
AGGGTGCCACCAGCAAATGGAAGGAATTCTAAATGAGATCGTAAATTTTG
TTTTACTTTTTCTCCAGGATCCTCATCCAAGAGTAAGGTATGCAGCCTGT
AATGCCGTGGGACAGATGGCTACAGATTTTGCACCTGGTTTCCAAAAGAA
ATTTCATGAGAAGGTGATTGCAGCTCTGCTGCAGACCATGGAAGACCAAG
GCAATCAACGTGTGCAGGCCCATGCAGCTGCTGCCCTCATTAACTTTACT
GAAGACTGTCCCAAGTCACTACTTATTCCATACTTGGATAATTTGGTGAA
ACATCTGCATTCCATTATGGTACTGAAGCTTCAAGAGCTGATTCAGAAAG
GCACCAAGTTAGTTTTGGAACAAGTTGTGACATCCATTGCATCAGTTGCC
GATACTGCAGAAGAAAAATTTGTCCCCTACTATGATTTATTTATGCCATC
ACGGAAGCACATCGTTGAGAATGCGGTTCAAAAAGAACTGAGACTTCTGA
GAGGAAAAACTATTGAATGCATTAGCCTCATTGGTCTGGCTGTTGGGAAG
GAAAAATTCATGCAGGATGCATCAGATGTGATGCAGCTTTTGTTAAAGAC
CCAGACAGACTTCAATGATATGGAAGATGATGATCCTCAGATCTCTTACA
TGATCTCAGCATGGGCCAGAATGTGCAAAATCCTTGGAAAAGAATTTCAG
CAATACCTTCCAGTGGTTATGGGGCCTTTAATGAAGACGGCTTCAATTAA
GCCCGAAGTAGCCCTTTTAGATACCCAAGACATGGAGAATATGAGTGATG
ATGATGGTTGGGAATTTGTGAACCTTGGAGATCAGCAAAGCTTTGGTATT
AAAACTGCAGGACTAGAAGAAAAATCAACTGCTTGCCAGATGTTGGTTTG
CTATGCTAAGGAGTTAAAGGAAGGCTTTGTGGAGTACACCGAACAGGTTG
TCAAACTGATGGTCCCTTTACTGAAATTTTATTTCCACGATGGTGTTCGA
GTGGCAGCAGCGGAATCCATGCCTCTTCTCCTGGAGTGTGCAAGAGTCCG
TGGTCCTGAGTACCTCACACAGATGTGGCATTTTATGTGTGATGCTCTAA
TTAAGGCCATTGGTACAGAACCAGATTCAGACGTCCTCTCAGAAATAATG
CATTCTTTTGCAAAGTGCATTGAAGTAATGGGAGATGGATGCCTTAATAA
TGAACACTTTGAAGAACTGGGAGGTATATTGAAAGCAAAGCTTGAAGAAC
ATTTTAAAAATCAAGAATTACGACAAGTTAAAAGACAAGATGAAGACTAT
GATGAACAGGTCGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTA
TATTCTGACCAAAGTGTCAGATATTTTACACTCAATATTCAGTAGCTACA
AAGAAAAGGTGTTACCATGGTTTGAACAGCTGCTTCCATTAATTGTCAAC
CTCATTTGTCCACATAGACCATGGCCAGACAGACAATGGGGATTATGCAT
CTTTGATGATGTCATAGAACACTGTAGTCCAGCCTCATTTAAATACGCAG
AATATTTCTTAAGACCAATGCTCCAATATGTATGTGACAACAGCCCAGAA
GTCAGGCAAGCAGCTGCATATGGCCTGGGAGTCATGGCACAGTACGGTGG
AGATAATTATCGCCCTTTTTGTACAGAAGCACTTCCCCTGCTGGTAAGAG
TTATTCAGTCTGCGGATTCTAAGACCAAAGAAAATGTCAATGCTACAGAG
AACTGCATCTCAGCAGTAGGGAAAATCATGAAGTTCAAGCCTGACTGTGT
AAACGTTGAAGAGGTCCTTCCACACTGGTTGTCTTGGCTTCCACTACATG
AAGATAAAGAAGAAGCTGTTCAGACTTTCAATTATCTGTGTGACCTGATT
GAAAGTAATCATCCAATTGTTCTTGGCCCAAACAATACCAATCTGCCCAA
AATATTTAGTATAATTGCGGAAGGAGAAATGCACGAGGCAATTAAACATG
AAGATCCTTGTGCCAAACGTCTGGCCAATGTCGTTCGCCAAGTACAGACT
TCTGGAGGACTGTGGACTGAGTGCATAGCACAGCTCAGTCCTGAGCAGCA
GGCCGCCATTCAGGAGCTCCTGAACTCTGCGTGAactagt

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSRAQASNSAVDMPE
DQVGKLEATENTISAMAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYEN
IPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDV
QTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLIDEDGNNQWPEGLKF
LFDSVSSQNVGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLVQCMQDQE
HPSIRTLSARATAAFILANEHNVALFKHFADLLPGFLQAVNDSCYQNDDS
VLKSLVEIADTVPKYLRPHLEATLQLSLKLCGDTSLNNMQRQLALEVIVT
LSETAAAMLRKHTNIVAQTIPQMLAMMVDLEEDEDWANADELEDDDFDSN
AVAGESALDRMACGLGGKLVLPMIKEHIMQMLQNPDWKYRHAGLMALSAI
GEGCHQQMEGILNEIVNFVLLFLQDPHPRVRYAACNAVGQMATDFAPGFQ
KKFHEKVIAALLQTMEDQGNQRVQAHAAAALINFTEDCPKSLLIPYLDNL
VKHLHSIMVLKLQELIQKGTKLVLEQVVTSIASVADTAEEKFVPYYDLFM
PSRKHIVENAVQKELRLLRGKTIECISLIGLAVGKEKFMQDASDVMQLLL
KTQTDFNDMEDDDPQISYMISAWARMCKILGKEFQQYLPVVMGPLMKTAS
IKPEVALLDTQDMENMSDDDGWEFVNLGDQQSFGIKTAGLEEKSTACQML
VCYAKELKEGFVEYTEQVVKLMVPLLKFYFHDGVRVAAAESMPLLLECAR
VRGPEYLTQMWHFMCDALIKAIGTEPDSDVLSEIMHSFAKCIEVMGDGCL
NNEHFEELGGILKAKLEEHFKNQELRQVKRQDEDYDEQVEESLQDEDDND
VYILTKVSDILHSIFSSYKEKVLPWFEQLLPLIVNLICPHRPWPDRQWGL
CIFDDVIEHCSPASFKYAEYFLRPMLQYVCDNSPEVRQAAAYGLGVMAQY
GGDNYRPFCTEALPLLVRVIQSADSKTKENVNATENCISAVGKIMKFKPD
CVNVEEVLPHWLSWLPLHEDKEEAVQTFNYLCDLIESNHPIVLGPNNTNL
PKIFSIIAEGEMHEAIKHEDPCAKRLANVVRQVQTSGGLWTECIAQLSPE
QQAAIQELLNSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7397
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human RANBP5 with N-terminal GST.; ; Estimated MW of expressed protein : 153999 Daltons