cDNA Clone RanBP5

• Synonyms: IPO5
• Plasmid: pEGFP-C1-RanBP5
• Parent Plasmid: pEGFPC1
• Expressed: GFP-RanBP5
• Genbank: NM_002271.4 GI:51873051
• Tag: GFP
• Species: Human

Sequence of Insert

gtcgacATGCCTGAGGATCAAGTTGGAAAACTAGAAGCAACAGAAAACAC
AATAAGCGCAATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGC
TCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGCAGAG
GAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACA
AGCCATCAGAAATACAACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCG
TTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATGAAGTCTATCCAGCA
CTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTAT
TCAGATGGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGG
CAGAACTGGCCAGGAATTTAATAGATGAGGATGGCAATAACCAGTGGCCC
GAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAATGTGGGACT
GCGGGAAGCTGCCCTTCACATTTTCTGGAACTTTCCTGGAATTTTTGGGA
ACCAGCAACAACACTATTTAGATGTCATCAAACGAATGTTAGTTCAGTGT
ATGCAAGATCAGGAACACCCGTCGATCAGGACGTTATCTGCTAGAGCTAC
AGCTGCATTTATACTTGCAAATGAGCATAATGTTGCTCTGTTCAAACATT
TTGCAGACTTGCTACCGGGATTCCTACAGGCGGTAAATGACTCGTGCTAC
CAGAATGATGATTCTGTCCTAAAATCCCTCGTTGAGATTGCAGATACTGT
TCCAAAGTATTTGCGTCCTCACTTGGAAGCAACTCTACAGCTAAGTCTAA
AGTTGTGTGGAGACACTAGCCTCAACAATATGCAACGCCAGCTTGCCCTT
GAAGTGATCGTCACCCTCTCTGAGACTGCAGCTGCTATGTTAAGAAAACA
TACCAATATTGTTGCACAGACTATTCCTCAGATGTTAGCAATGATGGTTG
ATTTGGAAGAAGATGAGGACTGGGCAAATGCAGATGAACTAGAAGATGAT
GATTTTGACAGCAATGCAGTTGCAGGCGAGAGTGCTCTAGATCGAATGGC
TTGCGGACTTGGTGGAAAGCTCGTTCTGCCGATGATCAAGGAACACATTA
TGCAAATGCTTCAAAATCCTGACTGGAAATACCGGCATGCAGGATTGATG
GCCTTATCTGCCATTGGTGAAGGGTGCCACCAGCAAATGGAAGGAATTCT
AAATGAGATCGTAAATTTTGTTTTACTTTTTCTCCAGGATCCTCATCCAA
GAGTAAGGTATGCAGCCTGTAATGCCGTGGGACAGATGGCTACAGATTTT
GCACCTGGTTTCCAAAAGAAATTTCATGAGAAGGTGATTGCAGCTCTGCT
GCAGACCATGGAAGACCAAGGCAATCAACGTGTGCAGGCCCATGCAGCTG
CTGCCCTCATTAACTTTACTGAAGACTGTCCCAAGTCACTACTTATTCCA
TACTTGGATAATTTGGTGAAACATCTGCATTCCATTATGGTACTGAAGCT
TCAAGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGA
CATCCATTGCATCAGTTGCCGATACTGCAGAAGAAAAATTTGTCCCCTAC
TATGATTTATTTATGCCATCACTGAAGCACATCGTTGAGAATGCGGTTCA
AAAAGAACTGAGACTTCTGAGAGGAAAAACTATTGAATGCATTAGCCTCA
TTGGTCTGGCTGTTGGGAAGGAAAAATTCATGCAGGATGCATCAGATGTG
ATGCAGCTTTTGTTAAAGACCCAGACAGACTTCAATGATATGGAAGATGA
TGATCCTCAGATCTCTTACATGATCTCAGCATGGGCCAGAATGTGCAAAA
TCCTTGGAAAAGAATTTCAGCAATACCTTCCAGTGGTTATGGGGCCTTTA
ATGAAGACGGCTTCAATTAAGCCCGAAGTAGCCCTTTTAGATACCCAAGA
CATGGAGAATATGAGTGATGATGATGGTTGGGAATTTGTGAACCTTGGAG
ATCAGCAAAGCTTTGGTATTAAAACTGCAGGACTAGAAGAAAAATCAACT
GCTTGCCAGATGTTGGTTTGCTATGCTAAGGAGTTAAAGGAAGGCTTTGT
GGAGTACACCGAACAGGTTGTCAAACTGATGGTCCCTTTACTGAAATTTT
ATTTCCACGATGGTGTTCGAGTGGCAGCAGCGGAATCCATGCCTCTTCTC
CTGGAGTGTGCAAGAGTCCGTGGTCCTGAGTATCTCACACAGATGTGGCA
TTTTATGTGTGATGCTCTAATTAAGGCCATTGGTACAGAACCAGATTCAG
ACGTCCTCTCAGAAATAATGCATTCTTTTGCAAAGTGCATTGAAGTAATG
GGAGATGGATGCCTTAATAATGAACACTTTGAAGAACTGGGAGGTATATT
GAAAGCAAAGCTTGAAGAACATTTTAAAAATCAAGAATTACGACAAGTTA
AAAGACAAGATGAAGACTATGATGAACAGGTCGAAGAGTCACTACAAGAT
GAGGATGATAATGATGTTTATATTCTGACCAAAGTGTCAGATATTTTACA
CTCAATATTCAGTAGCTACAAAGAAAAGGTGTTACCATGGTTTGAACAGC
TGCTTCCATTAATTGTCAACCTCATTTGTCCACATAGACCATGGCCAGAC
AGACAATGGGGATTATGCATCTTTGATGATGTCATAGAACACTGTAGTCC
AGCCTCATTTAAATACGCAGAATATTTCTTAAGACCAATGCTCCAATATG
TATGTGACAACAGCCCAGAAGTCAGGCAAGCAGCTGCATATGGCCTGGGA
GTCATGGCACAGTACGGTGGAGATAATTATCGCCCTTTTTGTACAGAAGC
ACTTCCCCTGCTGGTAAGAGTTATTCAGTCTGCGGATTCTAAGACCAAAG
AAAATGTCAATGCTACAGAGAACTGCATCTCAGCAGTAGGGAAAATCATG
AAGTTCAAGCCTGACTGTGTAAACGTTGAAGAGGTCCTTCCACACTGGTT
GTCTTGGCTTCCACTACATGAAGATAAAGAAGAAGCTGTTCAGACTTTCA
ATTATCTGTGTGACCTGATTGAAAGTAATCATCCAATTGTTCTTGGCCCA
AACAATACCAATCTGCCCAAAATATTTAGTATAATTGCGGAAGGAGAAAT
GCACGAGGCAATTAAACATGAAGATCCTTGTGCCAAACGTCTGGCCAATG
TCGTTCGCCAAGTACAGACTTCTGGAGGACTGTGGACTGAGTGCATAGCA
CAGCTCAGTCCTGAGCAGCAGGCCGCCATTCAGGAGCTCCTGAACTCTGC
GTGAactagtcgac

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDMPEDQVGKLEATENTISAMAAAAAEQQQFYLLLGNLLSPDNVVRKQ
AEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVY
PALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLIDEDGNNQ
WPEGLKFLFDSVSSQNVGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLV
QCMQDQEHPSIRTLSARATAAFILANEHNVALFKHFADLLPGFLQAVNDS
CYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLSLKLCGDTSLNNMQRQL
ALEVIVTLSETAAAMLRKHTNIVAQTIPQMLAMMVDLEEDEDWANADELE
DDDFDSNAVAGESALDRMACGLGGKLVLPMIKEHIMQMLQNPDWKYRHAG
LMALSAIGEGCHQQMEGILNEIVNFVLLFLQDPHPRVRYAACNAVGQMAT
DFAPGFQKKFHEKVIAALLQTMEDQGNQRVQAHAAAALINFTEDCPKSLL
IPYLDNLVKHLHSIMVLKLQELIQKGTKLVLEQVVTSIASVADTAEEKFV
PYYDLFMPSLKHIVENAVQKELRLLRGKTIECISLIGLAVGKEKFMQDAS
DVMQLLLKTQTDFNDMEDDDPQISYMISAWARMCKILGKEFQQYLPVVMG
PLMKTASIKPEVALLDTQDMENMSDDDGWEFVNLGDQQSFGIKTAGLEEK
STACQMLVCYAKELKEGFVEYTEQVVKLMVPLLKFYFHDGVRVAAAESMP
LLLECARVRGPEYLTQMWHFMCDALIKAIGTEPDSDVLSEIMHSFAKCIE
VMGDGCLNNEHFEELGGILKAKLEEHFKNQELRQVKRQDEDYDEQVEESL
QDEDDNDVYILTKVSDILHSIFSSYKEKVLPWFEQLLPLIVNLICPHRPW
PDRQWGLCIFDDVIEHCSPASFKYAEYFLRPMLQYVCDNSPEVRQAAAYG
LGVMAQYGGDNYRPFCTEALPLLVRVIQSADSKTKENVNATENCISAVGK
IMKFKPDCVNVEEVLPHWLSWLPLHEDKEEAVQTFNYLCDLIESNHPIVL
GPNNTNLPKIFSIIAEGEMHEAIKHEDPCAKRLANVVRQVQTSGGLWTEC
IAQLSPEQQAAIQELLNSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7370
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human RANBP5 with N-terminal EGFP.; ; Estimated MW of expressed protein : 153969 Daltons