cDNA Clone RanBP5

• Synonyms: IPO5
• Plasmid: pCMV5-HA3 RanBP5
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-RanBP5
• Genbank: NM_002271.4 GI:51873051
• Tag: HA
• Species: Human

Sequence of Insert

gtcgacATGCCTGAGGATCAAGTTGGAAAACTAGAAGCAACAGAAAACAC
AATAAGCGCAATGGCGGCGGCCGCGGCGGAGCAGCAACAGTTCTACCTGC
TCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGCAGAG
GAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACA
AGCCATCAGAAATACAACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCG
TTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATGAAGTCTATCCAGCA
CTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTAT
TCAGATGGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGG
CAGAACTGGCCAGGAATTTAATAGATGAGGATGGCAATAACCAGTGGCCC
GAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAATGTGGGACT
GCGGGAAGCTGCCCTTCACATTTTCTGGAACTTTCCTGGAATTTTTGGGA
ACCAGCAACAACACTATTTAGATGTCATCAAACGAATGTTAGTTCAGTGT
ATGCAAGATCAGGAACACCCGTCGATCAGGACGTTATCTGCTAGAGCTAC
AGCTGCATTTATACTTGCAAATGAGCATAATGTTGCTCTGTTCAAACATT
TTGCAGACTTGCTACCGGGATTCCTACAGGCGGTAAATGACTCGTGCTAC
CAGAATGATGATTCTGTCCTAAAATCCCTCGTTGAGATTGCAGATACTGT
TCCAAAGTATTTGCGTCCTCACTTGGAAGCAACTCTACAGCTAAGTCTAA
AGTTGTGTGGAGACACTAGCCTCAACAATATGCAACGCCAGCTTGCCCTT
GAAGTGATCGTCACCCTCTCTGAGACTGCAGCTGCTATGTTAAGAAAACA
TACCAATATTGTTGCACAGACTATTCCTCAGATGTTAGCAATGATGGTTG
ATTTGGAAGAAGATGAGGACTGGGCAAATGCAGATGAACTAGAAGATGAT
GATTTTGACAGCAATGCAGTTGCAGGCGAGAGTGCTCTAGATCGAATGGC
TTGCGGACTTGGTGGAAAGCTCGTTCTGCCGATGATCAAGGAACACATTA
TGCAAATGCTTCAAAATCCTGACTGGAAATACCGGCATGCAGGATTGATG
GCCTTATCTGCCATTGGTGAAGGGTGCCACCAGCAAATGGAAGGAATTCT
AAATGAGATCGTAAATTTTGTTTTACTTTTTCTCCAGGATCCTCATCCAA
GAGTAAGGTATGCAGCCTGTAATGCCGTGGGACAGATGGCTACAGATTTT
GCACCTGGTTTCCAAAAGAAATTTCATGAGAAGGTGATTGCAGCTCTGCT
GCAGACCATGGAAGACCAAGGCAATCAACGTGTGCAGGCCCATGCAGCTG
CTGCCCTCATTAACTTTACTGAAGACTGTCCCAAGTCACTACTTATTCCA
TACTTGGATAATTTGGTGAAACATCTGCATTCCATTATGGTACTGAAGCT
TCAAGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGA
CATCCATTGCATCAGTTGCCGATACTGCAGAAGAAAAATTTGTCCCCTAC
TATGATTTATTTATGCCATCACTGAAGCACATCGTTGAGAATGCGGTTCA
AAAAGAACTGAGACTTCTGAGAGGAAAAACTATTGAATGCATTAGCCTCA
TTGGTCTGGCTGTTGGGAAGGAAAAATTCATGCAGGATGCATCAGATGTG
ATGCAGCTTTTGTTAAAGACCCAGACAGACTTCAATGATATGGAAGATGA
TGATCCTCAGATCTCTTACATGATCTCAGCATGGGCCAGAATGTGCAAAA
TCCTTGGAAAAGAATTTCAGCAATACCTTCCAGTGGTTATGGGGCCTTTA
ATGAAGACGGCTTCAATTAAGCCCGAAGTAGCCCTTTTAGATACCCAAGA
CATGGAGAATATGAGTGATGATGATGGTTGGGAATTTGTGAACCTTGGAG
ATCAGCAAAGCTTTGGTATTAAAACTGCAGGACTAGAAGAAAAATCAACT
GCTTGCCAGATGTTGGTTTGCTATGCTAAGGAGTTAAAGGAAGGCTTTGT
GGAGTACACCGAACAGGTTGTCAAACTGATGGTCCCTTTACTGAAATTTT
ATTTCCACGATGGTGTTCGAGTGGCAGCAGCGGAATCCATGCCTCTTCTC
CTGGAGTGTGCAAGAGTCCGTGGTCCTGAGTATCTCACACAGATGTGGCA
TTTTATGTGTGATGCTCTAATTAAGGCCATTGGTACAGAACCAGATTCAG
ACGTCCTCTCAGAAATAATGCATTCTTTTGCAAAGTGCATTGAAGTAATG
GGAGATGGATGCCTTAATAATGAACACTTTGAAGAACTGGGAGGTATATT
GAAAGCAAAGCTTGAAGAACATTTTAAAAATCAAGAATTACGACAAGTTA
AAAGACAAGATGAAGACTATGATGAACAGGTCGAAGAGTCACTACAAGAT
GAGGATGATAATGATGTTTATATTCTGACCAAAGTGTCAGATATTTTACA
CTCAATATTCAGTAGCTACAAAGAAAAGGTGTTACCATGGTTTGAACAGC
TGCTTCCATTAATTGTCAACCTCATTTGTCCACATAGACCATGGCCAGAC
AGACAATGGGGATTATGCATCTTTGATGATGTCATAGAACACTGTAGTCC
AGCCTCATTTAAATACGCAGAATATTTCTTAAGACCAATGCTCCAATATG
TATGTGACAACAGCCCAGAAGTCAGGCAAGCAGCTGCATATGGCCTGGGA
GTCATGGCACAGTACGGTGGAGATAATTATCGCCCTTTTTGTACAGAAGC
ACTTCCCCTGCTGGTAAGAGTTATTCAGTCTGCGGATTCTAAGACCAAAG
AAAATGTCAATGCTACAGAGAACTGCATCTCAGCAGTAGGGAAAATCATG
AAGTTCAAGCCTGACTGTGTAAACGTTGAAGAGGTCCTTCCACACTGGTT
GTCTTGGCTTCCACTACATGAAGATAAAGAAGAAGCTGTTCAGACTTTCA
ATTATCTGTGTGACCTGATTGAAAGTAATCATCCAATTGTTCTTGGCCCA
AACAATACCAATCTGCCCAAAATATTTAGTATAATTGCGGAAGGAGAAAT
GCACGAGGCAATTAAACATGAAGATCCTTGTGCCAAACGTCTGGCCAATG
TCGTTCGCCAAGTACAGACTTCTGGAGGACTGTGGACTGAGTGCATAGCA
CAGCTCAGTCCTGAGCAGCAGGCCGCCATTCAGGAGCTCCTGAACTCTGC
GTGAactagtcgac

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMPEDQVGKLEATENTISAMAAAAAEQQQFYL
LLGNLLSPDNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAA
VLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIA
AELARNLIDEDGNNQWPEGLKFLFDSVSSQNVGLREAALHIFWNFPGIFG
NQQQHYLDVIKRMLVQCMQDQEHPSIRTLSARATAAFILANEHNVALFKH
FADLLPGFLQAVNDSCYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLSL
KLCGDTSLNNMQRQLALEVIVTLSETAAAMLRKHTNIVAQTIPQMLAMMV
DLEEDEDWANADELEDDDFDSNAVAGESALDRMACGLGGKLVLPMIKEHI
MQMLQNPDWKYRHAGLMALSAIGEGCHQQMEGILNEIVNFVLLFLQDPHP
RVRYAACNAVGQMATDFAPGFQKKFHEKVIAALLQTMEDQGNQRVQAHAA
AALINFTEDCPKSLLIPYLDNLVKHLHSIMVLKLQELIQKGTKLVLEQVV
TSIASVADTAEEKFVPYYDLFMPSLKHIVENAVQKELRLLRGKTIECISL
IGLAVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMISAWARMCK
ILGKEFQQYLPVVMGPLMKTASIKPEVALLDTQDMENMSDDDGWEFVNLG
DQQSFGIKTAGLEEKSTACQMLVCYAKELKEGFVEYTEQVVKLMVPLLKF
YFHDGVRVAAAESMPLLLECARVRGPEYLTQMWHFMCDALIKAIGTEPDS
DVLSEIMHSFAKCIEVMGDGCLNNEHFEELGGILKAKLEEHFKNQELRQV
KRQDEDYDEQVEESLQDEDDNDVYILTKVSDILHSIFSSYKEKVLPWFEQ
LLPLIVNLICPHRPWPDRQWGLCIFDDVIEHCSPASFKYAEYFLRPMLQY
VCDNSPEVRQAAAYGLGVMAQYGGDNYRPFCTEALPLLVRVIQSADSKTK
ENVNATENCISAVGKIMKFKPDCVNVEEVLPHWLSWLPLHEDKEEAVQTF
NYLCDLIESNHPIVLGPNNTNLPKIFSIIAEGEMHEAIKHEDPCAKRLAN
VVRQVQTSGGLWTECIAQLSPEQQAAIQELLNSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7262
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human RANBP5 with N-terminal HA tag.; ; Estimated MW of expressed protein : 127751 Daltons