cDNA Clone slit-robo-GAP 1

• Plasmid: pEBG6P-SRGAP1
• Parent Plasmid: pEBG6P
• Expressed: GST-Slit-Robo-GAP1 isoform 1
• Genbank: BC053903.1 GI:31753110
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGC
CGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAAC
AAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGAT
CTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCG
GAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTA
AGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAAC
TGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGC
AACCTTGAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGA
TAAGTGAGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTC
CAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGAT
GAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGC
TGAAAGAGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGAT
CCAGTCTTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGT
AAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAA
ATAAGCTAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAA
GCCACCAATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTT
AATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAA
GAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGC
TTAGACATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAA
GCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGT
TTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAG
CAGCCAGTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCG
CCTTGCCACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAG
CCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTT
TCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGT
CTCTGAAACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACC
AGCAGGAAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAA
GGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAG
GACCCTGGGAGAAGGTCATAGAGCTGAATATATGACTACAAGGCCTCCAA
ATGTTCCCCCTAAGCCCCAGAAACACAGGAAGTCCAGGCCCCGCTCACAG
TATAATACTAAGTTGTTTAATGGGGATTTGGAAACATTCGTCAAGGACTC
AGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATC
TCTATGGTCTTCAGCATCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTG
GAAGTCAATGATATTAAAAATTCATTTGAGAGAGGTGAAAATCCTTTGGC
TGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGC
TCTATTTCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAAC
GATCTGATTTCTTGTATCAGAATAGATAATCTCTATGAGAGGGCGCTTCA
CATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTGATGA
GGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAAT
ATGATGGACCCTTATAACCTGGCCATTTGCTTTGGCCCAACATTGATGCC
TGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGCACATGTGAATGAAA
TTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAA
GAGCTGGATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTG
CGACAGCCCATACAGTGAGCACGGTACATTGGAGGAAGTGGACCAAGATG
CTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGAAGCA
ATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAA
GAAGGGTGCCTCCCTGCTGCTGTATCACCGTGCATCTGAGGACTGGTGGG
AAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTCACCAGTATATAGTG
GTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGA
CAGTGAGGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGG
ACATGAACTCCCCGACAGACCGTCATCCTGACGGCTATTTAGCCAGGCAA
CGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGACCAG
TGATGGCCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAACTCCTCAG
TTGACCTAGGGTCCCCAAGCCTTGCCAGTCACCCCCGGGGCCTGCTGCAG
AACCGTGGCCTCAACAATGACAGTCCTGAGCGGAGGCGCAGGCCTGGCCA
TGGCAGCCTGACCAACATCAGCCGGCACGACTCCCTCAAGAAGATCGACA
GCCCTCCCATTAGAAGGTCCACGTCATCAGGGCAATACACGGGCTTCAAT
GACCACAAGCCACTGGACCCAGAGACAATTGCTCAGGATATTGAAGAAAC
GATGAACACAGCTTTGAATGAACTCCGAGAACTGGAGAGACAGAGCACAG
CAAAGCATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAGTGAAAAAC
TCTCCCACCCCTGCCACTTCCACGGAATCTCTCAGCCCTTTGCACAACGT
TGCCCTCAGGAGCTCCGAGCCTCAGATTCGACGTAGCACGAGCTCCTCCA
GTGACACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAATGGGCGTG
CAGCTGAAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTTCCAAAAAC
AAATCCTACCATAGGACCTGCCCCACCTCCCCAGGGTCCAACAGACAAGT
CATGCACAATGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSTPSRFKKDKEI
IAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEY
SRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKD
HATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYT
VMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRS
SVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLS
DLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRS
DKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQPVQAELMLRYQQLQ
SRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKS
TVSETYLSKPSIAKRRANQQETEQFYFMKLREYLEGSNLITKLQAKHDLL
QRTLGEGHRAEYMTTRPPNVPPKPQKHRKSRPRSQYNTKLFNGDLETFVK
DSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENP
LADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERA
LHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTL
MPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAGDD
YCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELS
FKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQK
ADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQRKRGEPPPPVRRPGR
TSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRRRP
GHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIE
ETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLH
NVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLP
KTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express SRGAP1 with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 151694 Daltons