cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCMV5-HA AS160A del 52-154 T642A
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-AS160A del 52-154 T642A
• Genbank: NM_014832
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgaattcactagtgattAAGGAGCCGCCCAGCTGCATTCAGGA
TGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGC
CCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGG
GGGTCGTGCCTGGACCACAGGACCACGGCCTGCCACGTTTTCCGCGCCAC
AGACCCCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTA
AAGCGGCCATGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCC
TTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGT
GACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGT
TCAGCCTGCACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGT
CCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTC
CCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTG
GCTTACCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGC
TTCCCTGAGCGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTT
TCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGG
GCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCAC
GTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGG
GCGATTTGAGATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAG
AAAAGAATTTTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTG
GATCACTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCA
GTATATTTGTTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGG
TAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGT
GCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCA
TAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGG
TGATACAGAGGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATC
TTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGA
ACTTGTGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACAC
ACGTGCACATCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCA
GAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAA
AGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAG
CTAACAGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCC
AACAGTCTTGCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGG
GACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAG
AGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCAC
CCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCA
GGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTG
ATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTA
AGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCG
CATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGC
AACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTC
TCTCCAACCATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGGGGAGGA
TGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCT
TGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAA
GAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGT
TAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAA
CTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATG
GAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGG
AGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGC
CTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAGGCAG
CTCACTGCTCAGCAGCATGCGATTCTTGTGGATTTAGGAAGGACGTTTCC
TACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGT
TTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGATACTGT
CAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGA
GCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCA
AGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTG
TCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGA
AAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGT
TTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATT
TTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTCAGCCTACTGAG
CAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTG
AGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAA
ATTATTACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTA
TGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCATATT
CCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAA
CTGAAAAGACAAAACATGGACCTCCTAGAAAAATTACAGGTAGCTCATAC
TAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGAGAGA
CCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTAT
CAAAAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGC
CAATTGTGACCTGTTGCTGAGAGACCTAAACTGCAACCCTAACAACAAAG
CCAAGATAGGAAATAAGCCATAAggatccgcaatcgaattcccgcggccg
c

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAANSLVIKEPPSCIQDEPFPHPLEPEPGV
SAQPGPGKPSDKRFRLWYVGGSCLDHRTTACHVFRATDPSQVPDVISSIR
QLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDC
MEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTD
THLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRR
VHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKS
VVLEKNFKDISPCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESL
VDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPR
AKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAK
QKTHVHIGEGPATISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIF
SRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQT
FPEEDSDSPQFRRRAHAFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSE
QCSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSP
SAMQQQDGLDRNELLPLSPLSPTMEEEPLVIFLSGEDDPEKIEERKKSKE
LRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKE
VLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLR
HRLPNKQQPPDISYKELLRQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQ
LSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDL
GFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWF
LTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFE
NIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQE
SSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLL
TRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALANCDLLLRDLNCNP
NNKAKIGNKP

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Protein sequence has the following mutations compared to NM_014832 : ; M1K, S387P and K958R.; Amino acids 52 to 154 were deleted during RT-PCR.; The cloned sequence represents a splice variant lacking amino acids 679-741. However, the majority of ESTs in the NCBI database represent this variant.; A further single amino acid deletion (G865) is also well documented in the NCBI EST database, e.g.; gi|33251358|gb|CF135914.1|, ; gi|32238979|gb|CD708349.1|, ; gi|32046370|emb|BX508803.1|.; ; Estimated MW of expressed protein : 131299 Daltons