cDNA Clone AXIN1

• Plasmid: pCMV5-HA AXIN1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-AXIN1
• Genbank: NM_003502
• Tag: HA
• Species: Human

Sequence of Insert

ggatctATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAG
TTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAAC
TGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGGGAAA
GGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGA
TCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCAT
ACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGG
ATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCT
GGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACT
CGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAAAGTAC
ATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAA
GAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGT
TTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTAT
CCCTCCTTCCTTAAGTCTGATATTTATTTGGAATATACGAGGACAGGCTC
GGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGA
AGGGCATATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAATGGAAG
TGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGG
AAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTCTCCT
CCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGG
GAGCCAGTCAACCCCTATTATGTCAATGCCGGCTATGCCCTGGCCCCAGC
CACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACA
CCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACAGGATC
CGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCG
GGTGCCCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAGGTCC
GCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCT
GTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCG
CGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCC
CAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGCACCACTTCCCG
CCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGA
GAACCCTGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACAC
CTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCAC
GTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACGGGAA
GCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACC
ACCGACACGTCCACCACCACGTCCACCACAGCACAGCCCGGCCCAAGGAG
CAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGG
CCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTG
TTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTG
GGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGC
CAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAA
TCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGG
ACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTC
CAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCT
CCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCAC
CCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGA
GGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGACAAGAT
CGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTT
GTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAG
GGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGG
GCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGG
GTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGA
GGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACTGAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPR
PASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAES
LHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRL
KLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTE
IQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYL
PTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSE
GREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDS
SVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKF
AEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKL
PPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPG
PGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHH
VHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNA
SDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIE
GEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHL
FIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRTRSQRKVGG
GSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYF
KKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7050
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 93009 Daltons