cDNA Clone AXIN1

• Plasmid: pGEX6P-AXIN1
• Parent Plasmid: pGEX6P1
• Expressed: GST-AXIN1
• Genbank: NM_003502
• Tag: GST
• Species: Human

Sequence of Insert

ggatctATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAG
TTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGGGAGAAC
TGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGGGAAA
GGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGA
TCTGGACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCAT
ACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGGATGACCAAGATGGG
ATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCT
GGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACT
CGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAAAGTAC
ATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAA
GAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGT
TTGACCAGGCCCAGACCGAAATCCAGGCCACTATGGAGGAAAACACCTAT
CCCTCCTTCCTTAAGTCTGATATTTATTTGGAATATACGAGGACAGGCTC
GGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGA
AGGGCATATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAATGGAAG
TGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGG
AAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTCTCCT
CCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGG
GAGCCAGTCAACCCCTATTATGTCAATGCCGGCTATGCCCTGGCCCCAGC
CACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTCCAGCGATGCAGACA
CCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACAGGATC
CGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCG
GGTGCCCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAGGTCC
GCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCT
GTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCG
CGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATCCATCGTCAGGGCCCC
CAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGCACCACTTCCCG
CCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGA
GAACCCTGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACAC
CTGGCCGCCAGTCGCCTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCAC
GTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACGGGAA
GCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACC
ACCGACACGTCCACCACCACGTCCACCACAGCACAGCCCGGCCCAAGGAG
CAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGG
CCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTG
TTGGCGCTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTG
GGCGTGGCGTGCAAAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGC
CAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAA
TCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGG
ACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTC
CAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCT
CCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCAC
CCAGCTCCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGA
GGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCAAGCAGAGGACAAGAT
CGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTT
GTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAG
GGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGG
GCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGG
GTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGA
GGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNIQEQGFPLDLGASFTED
APRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLG
YEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWF
ACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIK
GCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPK
VCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQ
KLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSAN
DSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLP
HIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRME
EEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPES
ILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPK
SGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPH
SHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEV
PGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLS
LEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEK
RASRAPSKQRTRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAV
TLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKII
GKVEKVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU7049
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 118473 Daltons