cDNA Clone FASCIN1

• Plasmid: pEBG6P-FASCIN1 Thr346Ala
• Parent Plasmid: pEBG6P
• Expressed: GST-FASCIN1 Thr346Ala
• Genbank: NM_003088
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGACCGCCAACGGCACAGCCGAGGCGGTGCAGATCCAGTTCGG
CCTCATCAACTGCGGCAACAAGTACCTGACGGCCGAGGCGTTCGGGTTCA
AGGTGAACGCGTCCGCCAGCAGCCTGAAGAAGAAGCAGATCTGGACGCTG
GAGCAGCCCCCTGACGAGGCGGGCAGCGCGGCCGTGTGCCTGCGCAGCCA
CCTGGGCCGCTACCTGGCGGCGGACAAGGACGGCAACGTGACCTGCGAGC
GCGAGGTGCCCGGTCCCGACTGCCGTTTCCTCATCGTGGCGCACGACGAC
GGTCGCTGGTCGCTGCAGTCCGAGGCGCACCGGCGCTACTTCGGCGGCAC
CGAGGACCGCCTGTCCTGCTTCGCGCAGACGGTGTCCCCCGCCGAGAAGT
GGAGCGTGCACATCGCCATGCACCCTCAGGTCAACATCTACAGCGTCACC
CGTAAGCGCTACGCGCACCTGAGCGCGCGGCCGGCCGACGAGATCGCCGT
GGACCGCGACGTGCCCTGGGGCGTCGACTCGCTCATCACCCTCGCCTTCC
AGGACCAGCGCTACAGCGTGCAGACCGCCGACCACCGCTTCCTGCGCCAC
GACGGGCGCCTGGTGGCGCGCCCCGAGCCGGCCACTGGCTACACGCTGGA
GTTCCGCTCCGGCAAGGTGGCCTTCCGCGACTGCGAGGGCCGTTACCTGG
CGCCGTCGGGGCCCAGCGGCACGCTCAAGGCGGGCAAGGCCACCAAGGTG
GGCAAGGACGAGCTCTTTGCTCTGGAGCAGAGCTGCGCCCAGGTCGTGCT
GCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGTATGGACCTGT
CTGCCAATCAGGACGAGGAGACCGACCAGGAGACCTTCCAGCTGGAGATC
GACCGCGACACCAAAAAGTGTGCCTTCCGTACCCACACGGGCAAGTACTG
GACGCTGACGGCCACCGGGGGCGTGCAGTCCACCGCCTCCAGCAAGAATG
CCAGCTGCTACTTTGACATCGAGTGGCGTGACCGGCGCATCACACTGAGG
GCGTCCAATGGCAAGTTTGTGACCTCCAAGAAGAATGGGCAGCTGGCCGC
CTCGGTGGAGACAGCAGGGGACTCAGAGCTCTTCCTCATGAAGCTCATCA
ACCGCCCCATCATCGTGTTCCGCGGGGGGCATGGCTTCATCGGCTGCCGC
AAGGTCACGGGCACCCTGGACGCCAACCGCTCCAGCTATGACGTCTTCCA
GCTGGAGTTCAACGATGGCGCCTACAACATCAAAGACTCCACAGGCAAAT
ACTGGACGGTGGGCAGTGACTCCGTGGTCACCAGCAGCGGCGACACTCCT
GTGGACTTCTTCTTCGAGTTCTGCGACTATAACAAGGTGGCCATCAAGGT
GGGCGGGCGCTACCTGAAGGGCGACCACGCAGGCGTCCTGAAGGCCTCGG
CGGAAACCGTGGACCCCGCCTCGCTCTGGGAGTACTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMTANGTAEAVQIQ
FGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLR
SHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFG
GTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEI
AVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYT
LEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQV
VLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGK
YWTLTATGGVQSTASSKNASCYFDIEWRDRRIALRASNGKFVTSKKNGQL
AASVETAGDSELFLMKLINRPIIVFRGGHGFIGCRKVTGTLDANRSSYDV
FQLEFNDGAYNIKDSTGKYWTVGSDSVVTSSGDTPVDFFFEFCDYNKVAI
KVGGRYLKGDHAGVLKASAETVDPASLWEY

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7043
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Thr346Ala human FASCIN1 homolog with N-terminal GST (contains precission protease site); Estimated MW of expressed protein : 81886 Daltons